Wang L, Kunze R
Institut für Genetik und Microbiologie, Ludwig-Maximilians-Universität München, Germany.
Plant J. 1998 Feb;13(4):577-82. doi: 10.1046/j.1365-313x.1998.00060.x.
The authors have determined the C-methylation pattern of the non-autonomous transposable element Ds-cy, which is an epigenetically inactivated, transcriptionally silent derivative of the maize Activator (Ac) element. Like Ac, Ds-cy is hypermethylated at the 3-end. However, in Ds-cy the 5-end is also hypermethylated, including all subterminal binding sites for the Ac-encoded transposase protein. As Ds-cy transposes in the presence of an active Ac in the genome, the authors conclude that methylation of the 5-end TPase binding sites does not interfere with transposition, but correlates with inactivity of the Ac promoter. the authors discuss the implications of these results for the chromatid selectivity of Ac/Ds transposition and the mechanism of Ac-induced chromosome breakage events.
作者已经确定了非自主转座元件Ds-cy的C-甲基化模式，它是玉米激活子（Ac）元件的一种表观遗传失活、转录沉默的衍生物。与Ac一样，Ds-cy在3'端发生了高度甲基化。然而，在Ds-cy中，5'端也发生了高度甲基化，包括Ac编码的转座酶蛋白的所有亚末端结合位点。由于Ds-cy在基因组中存在活性Ac的情况下进行转座，作者得出结论，5'端转座酶结合位点的甲基化并不干扰转座，但与Ac启动子的无活性相关。作者讨论了这些结果对Ac/Ds转座的染色单体选择性以及Ac诱导的染色体断裂事件机制的影响。
