Leu J Y, Sun Y H, Lai Y K, Chen J
Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan.
Mol Gen Genet. 1992 Jun;233(3):411-8. doi: 10.1007/BF00265438.
Sequences sharing homology to the transposable element Activator (Ac) are prevalent in the maize genome. A cryptic Ac-like DNA, cAc-11, was isolated from the maize inbred line 4Co63 and sequenced. Cryptic Ac-11 has over 90% homology to known Ac sequences and contains an 11 bp inverted terminal repeat flanked by an 8 bp target site duplication, which are characteristics of Ac and Dissociation (Ds) transposable elements. Unlike the active Ac element, which encodes a transposase, the corresponding sequence in cAc-11 has no significant open reading frame. A 44 bp tandem repeat was found at one end of cAc-11, which might be a result of aberrant transposition. The sequence data suggest that cAc-11 may represent a remnant of an Ac or a Ds element. Sequences homologous to cAc-11 can be detected in many maize inbred lines. In contrast to canonical Ac elements, cAc-11 DNA in the maize genome is hypermethylated and does not transpose even in the presence of an active Ac element.
与转座因子激活子（Ac）具有同源性的序列在玉米基因组中普遍存在。从玉米自交系4Co63中分离出一个隐秘的类Ac DNA，即cAc-11，并进行了测序。隐秘的Ac-11与已知的Ac序列具有超过90%的同源性，并且包含一个11 bp的反向末端重复序列，两侧是一个8 bp的靶位点重复序列；这些都是Ac和解离（Ds）转座因子的特征。与编码转座酶的活性Ac元件不同，cAc-11中的相应序列没有明显的开放阅读框。在cAc-11的一端发现了一个44 bp的串联重复序列，这可能是异常转座的结果。序列数据表明，cAc-11可能代表一个Ac或Ds元件的残余。在许多玉米自交系中都能检测到与cAc-11同源的序列。与典型的Ac元件不同，玉米基因组中的cAc-11 DNA发生了高度甲基化，即使在存在活性Ac元件的情况下也不会转座。
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