Yoshida S, Taniguchi M, Hirata A, Shiosaka S
Division of Structural Cell Biology, Nara Institute of Technology, 8916-5 Talayama Ikoma, Nara 630-1, Japan.
Gene. 1998 Jun 15;213(1-2):9-16. doi: 10.1016/s0378-1119(98)00232-7.
Neuropsin is a serine protease which is thought to function in a variety of tissues including the brain and skin. This protease has been shown to have important roles in neural plasticity in mice. Here we have cloned a cDNA and analyzed the gene for human neuropsin by polymerase chain reaction-based strategies. The cDNA had 72% identity to mouse neuropsin. The deduced amino acid sequence showed 72% identity to mouse neuropsin. Key amino acid residues for the enzyme activity and all cysteine residues were conserved between human and mouse neuropsin. The gene for human neuropsin had six exons and five introns, and the gene organization is similar to trypsin-type serine proteases. The mRNA was expressed in primary cultures of keratinocytes.
神经蛋白酶是一种丝氨酸蛋白酶，被认为在包括大脑和皮肤在内的多种组织中发挥作用。这种蛋白酶已被证明在小鼠的神经可塑性中具有重要作用。在这里，我们通过基于聚合酶链反应的策略克隆了一个cDNA并分析了人类神经蛋白酶的基因。该cDNA与小鼠神经蛋白酶有72%的同一性。推导的氨基酸序列与小鼠神经蛋白酶有72%的同一性。人类和小鼠神经蛋白酶之间酶活性的关键氨基酸残基以及所有半胱氨酸残基都是保守的。人类神经蛋白酶的基因有六个外显子和五个内含子，其基因结构与胰蛋白酶型丝氨酸蛋白酶相似。该mRNA在角质形成细胞的原代培养物中表达。
