Toro F, Conesa A, Garcia A, Bianco N E, De Sanctis J B
Faculty of Medicine, Universidad Central de Venezuela, Apartado 50109, Caracas, 1050-A, Venezuela.
Clin Immunol Immunopathol. 1998 Aug;88(2):169-75. doi: 10.1006/clin.1998.4564.
To evaluate the oxidative burst in hepatitis C virus (HCV) infection, intracellular hydrogen peroxide (H2O2) production of polymorphonuclear (PMN) cells isolated from 15 chronic HCV-infected patients and 11 controls was assessed by flow cytometry in a time kinetic. Under nonstimulated and phorbol myristate acetate (PMA)-stimulated conditions, H2O2 production was higher in HCV-infected patients than in controls (P <0.05) at the time points of 20, 30, and 40 min. A positive correlation between H2O2 production by PMA-stimulated cells and serum levels of alanine aminotransferase and aspartate aminotransferase was found in the HCV-infected patients (r = 0.877, P <0.01 and r = 0.9351, P <0.001, respectively). RT-PCR analysis of purified mononuclear (MN) and PMN cells from HCV-infected patients revealed the presence of HCV RNA in 60% of MN and 27% of PMN cell samples. These results suggest that a functional alteration of PMN cells is manifested in this chronic viral infection which may represent an additional factor in the development of liver lesions.
为评估丙型肝炎病毒（HCV）感染中的氧化爆发，采用流式细胞术对15例慢性HCV感染患者和11例对照者分离的多形核（PMN）细胞的细胞内过氧化氢（H2O2）生成进行了时间动力学评估。在非刺激和佛波酯（PMA）刺激条件下，在20、30和40分钟时间点，HCV感染患者的H2O2生成高于对照组（P<0.05）。在HCV感染患者中，PMA刺激细胞产生的H2O2与血清丙氨酸氨基转移酶和天冬氨酸氨基转移酶水平呈正相关（r = 0.877，P<0.01；r = 0.9351，P<0.001）。对HCV感染患者纯化的单核（MN）细胞和PMN细胞进行RT-PCR分析，结果显示60%的MN细胞样本和27%的PMN细胞样本中存在HCV RNA。这些结果表明，在这种慢性病毒感染中PMN细胞出现功能改变，这可能是肝脏病变发展的一个额外因素。
