Mendoza L, Alvarez-Buylla E R
Lab. de Genética Molecular y Evolución, UNAM, Coyoacán, D.F., Mexico.
J Theor Biol. 1998 Jul 27;193(2):307-19. doi: 10.1006/jtbi.1998.0701.
We present a network model and its dynamic analysis for the regulatory relationships among 11 genes that participate in Arabidopsis thaliana flower morphogenesis. The topology of the network and the relative strengths of interactions among these genes were based from published genetic and molecular data, mainly relying on mRNA expression patterns under wild type and mutant backgrounds. The network model is made of binary elements and we used a particular dynamic implementation for the network that we call semi-synchronic. Using this method the network reaches six attractors; four of them correspond to observed patterns of gene expression found in the floral organs of Arabidopsis (sepals, petals, stamens and carpels) as predicted by the ABC model of flower morphogenesis. The fifth state corresponds to cells that are not competent to flowering, and the sixth attractor predicted by the model is never found in wild-type plants, but it could be induced experimentally. We discuss the biological implications and the potential use of this network modeling approach to integrate functional data of regulatory genes of plant development.
我们提出了一个网络模型及其动力学分析，用于研究参与拟南芥花形态发生的11个基因之间的调控关系。该网络的拓扑结构以及这些基因之间相互作用的相对强度基于已发表的遗传和分子数据，主要依赖于野生型和突变背景下的mRNA表达模式。该网络模型由二元元件组成，我们对该网络使用了一种特殊的动力学实现方式，称为半同步。使用这种方法，网络达到六个吸引子；其中四个对应于花形态发生的ABC模型所预测的在拟南芥花器官（萼片、花瓣、雄蕊和心皮）中发现的基因表达模式。第五种状态对应于无开花能力的细胞，该模型预测的第六个吸引子在野生型植物中从未发现，但可以通过实验诱导产生。我们讨论了这种网络建模方法在整合植物发育调控基因功能数据方面的生物学意义和潜在用途。
