Igaz P, Tóth S, Madurka I, Falus A
Genetikai, Sejt- és Immunbiológiai Intézet, Semmelweis Orvostudományi Egyetem, Budapest.
Orv Hetil. 1998 Jul 19;139(29):1741-4.
Interleukin-6 is a multifunctional cytokine participating in the regulation of several immunologic and other cell-physiological phenomena. It acts via a receptor consisting of two components, that besides the ligand-specific chain also contains a second component of 130 kD (gp 130). The soluble form of the ligand-specific component of this receptor was shown to occur physiologically in body fluids and -following the binding of interleukin-6-to be capable of associating with the membrane-bound receptor component and inducing signal-transduction. We studied the possible differences between the effects of interleukin-6 exerted via membrane-bound or soluble receptors on HepG2 human hepatoma and primary rat hepatocyte cultures. We used two methods to study the action of interleukin-6: the mRNA expression of the protooncogene junB as an early marker, and the protein production of fibrinogen as a late one. The effect of interleukin-6 on both cell types examined with both methods used was lower via the soluble than the membrane-bound receptor. In addition, the soluble receptors alone (without interleukin-6) could induce the expression of the junB gene. Considering the wide-spread biological and pathological activities of interleukin-6 these phenomena could have some role in the pathogenesis of some diseases.
白细胞介素-6是一种多功能细胞因子，参与多种免疫及其他细胞生理现象的调节。它通过一种由两个成分组成的受体发挥作用，该受体除了配体特异性链外，还包含一个130kD的第二成分（gp130）。已证明该受体配体特异性成分的可溶性形式在生理状态下存在于体液中，并且在白细胞介素-6结合后，能够与膜结合受体成分结合并诱导信号转导。我们研究了白细胞介素-6通过膜结合受体或可溶性受体对人肝癌细胞系HepG2和原代大鼠肝细胞培养物产生的影响之间可能存在的差异。我们使用两种方法研究白细胞介素-6的作用：将原癌基因junB的mRNA表达作为早期标志物，将纤维蛋白原的蛋白产生作为晚期标志物。使用这两种方法检测时，白细胞介素-6对两种细胞类型的作用通过可溶性受体比通过膜结合受体更低。此外，单独的可溶性受体（无白细胞介素-6）可诱导junB基因的表达。鉴于白细胞介素-6广泛的生物学和病理学活性，这些现象可能在某些疾病的发病机制中发挥一定作用。
