Millner S N, Taber C A
Clin Chim Acta. 1976 Aug 16;71(1):67-74. doi: 10.1016/0009-8981(76)90276-x.
Estriol in pregnancy urine is determined by acid hydrolysis of steroid conjugates, followed by ether and aqueous sodium hydroxide extraction and gas chromatography. Derivatives are formed within the injection port of the gas chromatograph using tetramethylammonium hydroxide. Several samples may be processed simultaneously. Hydrolysis and extraction of a specimen are done in a single culture tube; for larger numbers of specimens, a Paton-Brown extractor may be used. With a two-column gas chromatograph, results on two specimens are available within one hour; twelve analyses may be completed within two hours.
妊娠尿中雌三醇的测定方法是，先对类固醇结合物进行酸水解，然后用乙醚和氢氧化钠水溶液萃取，再进行气相色谱分析。在气相色谱仪的进样口使用氢氧化四甲铵形成衍生物。几个样品可同时进行处理。标本的水解和萃取在单个培养管中进行；对于大量标本，可使用帕顿-布朗萃取器。使用双柱气相色谱仪，一小时内可得出两个标本的结果；两小时内可完成十二次分析。
