Gotelli G R, Kabra P M, Marton L J
Clin Chem. 1977 Feb;23(2 PT. 1):165-8.
Placental estriol in urine can be determined by rapid enzymatic hydrolysis, extraction with neutral XAD-2 resin, and quantitation by gas-liquid chromatography, with equilenin as the internal standard. Glucose, methenamine mandelate, hydrochlorothiazide, phenolphthalein, or salicylate do not interfere. Analytical recovery of estriol added to urine was 92-108%. Day-to-day precison (CV), established by 15 repilicate analyses at a mean concentration of 21.3 mg/liter, was 6.6%.
尿中胎盘雌三醇可通过快速酶水解、用中性XAD - 2树脂萃取以及以马萘雌酮为内标物的气液色谱法定量来测定。葡萄糖、乌洛托品扁桃酸盐、氢氯噻嗪、酚酞或水杨酸盐不产生干扰。添加到尿中的雌三醇的分析回收率为92 - 108%。通过在平均浓度为21.3毫克/升的情况下进行15次重复分析确定的日间精密度（CV）为6.6%。
