Wickings E J, Nieschlag E
Clin Chim Acta. 1976 Sep 20;71(3):439-43. doi: 10.1016/0009-8981(76)90095-4.
In order to investigate the stability of testosterone (T) and androstenedione (Adione) in blood and plasma samples following study was undertaken. 1. Pooled blood samples were left unseparated at room temperature for varying time periods of up to 3 days before being centrifuged. Subsequent plasma T and Adione values measured by radioimmunoassay showed only small, insignificant variations. 2. Plasma, initially separated from the pooled blood sample, was left at room temperature for up to 24 h. There was no significant change in the levels of T and Adione measured over this period. There was also good correlation between steroid concentrations measured in blood and plasma. 3. T and Adione were measured in a pooled plasma sample after repeated freezing and thawing of the sample. All T values were well within the intraassay coefficient of variation; all Adione values were within the precision limits of the assay, although there was a considerable spread in individual values. It is concluded that the various handling procedures of blood or plasma have no significant effect on T and Adione levels.
为了研究睾酮（T）和雄烯二酮（Adione）在血液和血浆样本中的稳定性，进行了以下研究。1. 将混合血液样本在室温下不分离放置长达3天的不同时间段，然后进行离心。随后通过放射免疫测定法测量的血浆T和Adione值仅显示出微小的、无显著意义的变化。2. 最初从混合血液样本中分离出的血浆在室温下放置长达24小时。在此期间测量的T和Adione水平没有显著变化。血液和血浆中测量的类固醇浓度之间也有良好的相关性。3. 在对混合血浆样本进行反复冻融后测量T和Adione。所有T值均在测定内变异系数范围内；所有Adione值均在测定的精密度限度内，尽管个别值有相当大的离散度。得出的结论是，血液或血浆的各种处理程序对T和Adione水平没有显著影响。
