Wang I J, Shih Y F, Tseng H S, Huang S H, Lin L L, Hung P T
Department of Ophthalmology, National Taiwan University Hospital, Taipei, Republic of China.
J Ocul Pharmacol Ther. 1998 Aug;14(4):337-43. doi: 10.1089/jop.1998.14.337.
Atropine was found to be effective in arresting the progression of myopia. However, the actual mechanism is still unclear. Thus, we tried to investigate the in vivo effect of atropine on the proliferation of scleral chondrocytes in chicks of form-deprivation myopia. Twenty chicks were equally divided into 4 groups which included intravitreal injection of normal saline (IVN), IVN with goggling (IVNG), intravitreal injection of atropine (1%) (IVA), and IVA with goggling (IVAG) groups. Intravenous injection of bromodeoxyuridine (BrdU) (30 mg/kg) from subaxillary vein was performed 2 hours before being sacrificed. The eyeballs were then fixed in 10% neutral-buffered formalin at 4 degrees C. Standard BrdU immunohistochemical staining was performed. The BrdU labeling index was obtained from the average of positive labelings of BrdU in scleral chondrocytes for every 100 counting cells in posterior poles and anterior scleral margins by two experienced technicians. The BrdU index on the anterior scleral margin of the IVAG group was less than that of the IVNG group. The index on the anterior scleral margin of the IVNG group was higher than the IVN group. Although the index on the posterior poles of the IVNG group was also higher than the IVN group, it was statistically not different. Also, no statistical difference was found between IVN and IVA on the anterior scleral margins or posterior poles. The index was significantly different on the anterior scleral margins, but not on the posterior pole among each group. Therefore, intravitreal injection of atropine could inhibit the proliferation of chondrocytes on the anterior margins of sclera, but not the posterior poles in form-deprivation myopia.
研究发现阿托品对抑制近视进展有效。然而，其实际机制仍不清楚。因此，我们试图研究阿托品对形觉剥夺性近视雏鸡巩膜软骨细胞增殖的体内作用。将20只雏鸡平均分为4组，包括玻璃体内注射生理盐水（IVN）组、玻璃体内注射生理盐水并佩戴眼罩（IVNG）组、玻璃体内注射1%阿托品（IVA）组和玻璃体内注射阿托品并佩戴眼罩（IVAG）组。在处死前2小时，从腋静脉静脉注射溴脱氧尿苷（BrdU）（30mg/kg）。然后将眼球固定于4℃的10%中性缓冲福尔马林中。进行标准的BrdU免疫组织化学染色。BrdU标记指数由两名经验丰富的技术人员通过对后极和巩膜前缘每100个计数细胞中巩膜软骨细胞BrdU阳性标记的平均值获得。IVAG组巩膜前缘的BrdU指数低于IVNG组。IVNG组巩膜前缘的指数高于IVN组。虽然IVNG组后极的指数也高于IVN组，但差异无统计学意义。此外，IVN组和IVA组在巩膜前缘或后极均未发现统计学差异。各组之间巩膜前缘的指数有显著差异，但后极无显著差异。因此，玻璃体内注射阿托品可抑制形觉剥夺性近视巩膜前缘软骨细胞的增殖，但不能抑制后极软骨细胞的增殖。
