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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Morris B J, Nixon R L, Johnston C I

Clin Exp Pharmacol Physiol. 1976 Jan-Feb;3(1):37-47. doi: 10.1111/j.1440-1681.1976.tb00589.x.

Glomeruli free from renal tubules and cell debris were isolated in large numbers from rat kidney cortex by a rapid, simple process of graded sieving on stainless steel meshes. 2. Glomeruli were viable, had fragments of the arterioles attached and contained renin. 3. Glomeruli, when incubated, released renin into the medium. Using a super-fusion technique this was shown to be time dependent until a basal secretion level was achieved. 4. Release of renin was directly proportional to the number of glomeruli and could be stimulated by isoprenaline, adrenaline and noradrenaline in order of the potency of their action on beta-adrenoreceptors. 5. Isolated glomeruli were therefore established as a useful model system for studying the release of renin by direct influences on the juxtaglomerular cells where haemodynamic, tubular and extrarenal influences are removed.

通过在不锈钢筛网上进行快速、简单的分级筛分过程，从大鼠肾皮质中大量分离出不含肾小管和细胞碎片的肾小球。2. 肾小球具有活力，附着有小动脉碎片并含有肾素。3. 肾小球在孵育时会将肾素释放到培养基中。使用超级融合技术表明，在达到基础分泌水平之前，这是时间依赖性的。4. 肾素的释放与肾小球数量成正比，并且可以被异丙肾上腺素、肾上腺素和去甲肾上腺素按其对β-肾上腺素能受体作用的效力顺序刺激。5. 因此，分离的肾小球被确立为一种有用的模型系统，用于通过直接影响肾小球旁细胞来研究肾素的释放，在该模型中去除了血流动力学、肾小管和肾外影响。

Morris B J, Nixon R L, Johnston C I

Clin Exp Pharmacol Physiol. 1976 Jan-Feb;3(1):37-47. doi: 10.1111/j.1440-1681.1976.tb00589.x.

Glomeruli free from renal tubules and cell debris were isolated in large numbers from rat kidney cortex by a rapid, simple process of graded sieving on stainless steel meshes. 2. Glomeruli were viable, had fragments of the arterioles attached and contained renin. 3. Glomeruli, when incubated, released renin into the medium. Using a super-fusion technique this was shown to be time dependent until a basal secretion level was achieved. 4. Release of renin was directly proportional to the number of glomeruli and could be stimulated by isoprenaline, adrenaline and noradrenaline in order of the potency of their action on beta-adrenoreceptors. 5. Isolated glomeruli were therefore established as a useful model system for studying the release of renin by direct influences on the juxtaglomerular cells where haemodynamic, tubular and extrarenal influences are removed.

通过在不锈钢筛网上进行快速、简单的分级筛分过程，从大鼠肾皮质中大量分离出不含肾小管和细胞碎片的肾小球。2. 肾小球具有活力，附着有小动脉碎片并含有肾素。3. 肾小球在孵育时会将肾素释放到培养基中。使用超级融合技术表明，在达到基础分泌水平之前，这是时间依赖性的。4. 肾素的释放与肾小球数量成正比，并且可以被异丙肾上腺素、肾上腺素和去甲肾上腺素按其对β-肾上腺素能受体作用的效力顺序刺激。5. 因此，分离的肾小球被确立为一种有用的模型系统，用于通过直接影响肾小球旁细胞来研究肾素的释放，在该模型中去除了血流动力学、肾小管和肾外影响。