从新型隐球菌和其他致病性酵母中快速简便提取和保存DNA的方法。

Rapid and easy method to extract and preserve DNA from Cryptococcus neoformans and other pathogenic yeasts.

作者信息

Sansinforiano M E, Padilla J A, Hermoso de Mendoza J, Hermoso de Mendoza M, Fernandez-Garcia J L, Martínez-Tranćon M, Rabasco A, Parejo J C

机构信息

Unidad Docente de Genética y Mejora Animal, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain.

出版信息

Mycoses. 1998 May-Jun;41(5-6):195-8. doi: 10.1111/j.1439-0507.1998.tb00323.x.

DOI:10.1111/j.1439-0507.1998.tb00323.x

PMID:9715632

Abstract

The mucopolysaccharide capsule of Cryptococcus neoformans and other pathogenic yeasts prevent the extraction of DNA from these important zoonotic agents. We report that the use of a lysis buffer containing a high concentration of urea is an easy, efficient and time-saving technique to obtain high yields of good-quality DNA for molecular diagnosis. The use of urea also prevents the degradation of DNA during storage of samples at room temperature for up to 6 months.

摘要

新型隐球菌及其他致病性酵母的黏多糖荚膜会阻碍从这些重要的人畜共患病原体中提取DNA。我们报告称，使用含有高浓度尿素的裂解缓冲液是一种简便、高效且省时的技术，可获得用于分子诊断的高质量DNA高产率。使用尿素还可防止样品在室温下储存长达6个月期间DNA的降解。