Spear G T, Sha B E, Saarloos M N, Benson C A, Rydman R, Massad L S, Gilmore R, Landay A L
Department of Immunology/Microbiology, Rush Medical College, Chicago, Illinois, USA.
J Acquir Immune Defic Syndr Hum Retrovirol. 1998 Aug 15;18(5):454-9. doi: 10.1097/00042560-199808150-00006.
In this cross-sectional study, 53 cervicovaginal lavage samples (CVL) from 41 women were analyzed for the chemokines interleukin-8 (IL-8), regulated-on-activation normal T-expressed and secreted (RANTES) factor, and macrophage inflammatory protein-1alpha (MIP-1alpha) by enzyme-linked immunosorbent assay (ELISA). IL-8 was detected in 81% of CVL, whereas RANTES was detected in 32%, and MIP-1alpha in 15% of the CVL. The mean levels of IL-8, RANTES, and MIP-1alpha in positive samples were 396 pg/ml, 102 pg/ml, and 34 pg/ml, respectively. IL-8 levels correlated positively with IL-1beta and IgG in a subset of CVL samples. RANTES levels correlated positively with complement protein levels. Additionally, the levels of RANTES, but not MIP-1alpha, reached levels reported in previous studies of the effects of beta chemokines to inhibit HIV replication. These results suggest that measuring chemokines in CVL specimens can provide important information regarding immune responses in the genital tract.
在这项横断面研究中，通过酶联免疫吸附测定（ELISA）对来自41名女性的53份宫颈阴道灌洗样本（CVL）进行了趋化因子白细胞介素-8（IL-8）、正常T细胞激活后表达和分泌的调节因子（RANTES）以及巨噬细胞炎性蛋白-1α（MIP-1α）的分析。81%的CVL样本检测到IL-8，32%检测到RANTES，15%检测到MIP-1α。阳性样本中IL-8、RANTES和MIP-1α的平均水平分别为396 pg/ml、102 pg/ml和34 pg/ml。在一部分CVL样本中，IL-8水平与IL-1β和IgG呈正相关。RANTES水平与补体蛋白水平呈正相关。此外，RANTES的水平而非MIP-1α的水平达到了先前关于β趋化因子抑制HIV复制作用研究中所报道的水平。这些结果表明，检测CVL样本中的趋化因子可为生殖道免疫反应提供重要信息。
