Frim J, Kruuv J, Frey H E, Raaphorst G P
Cryobiology. 1976 Aug;13(4):475-83. doi: 10.1016/0011-2240(76)90104-8.
Unprotected, mammalian cells in plateau phase are at least a factor of four times more sensitive to freeze-thaw damage than exponential-phase cells. The former suffer about 15-20% more sublethal damage after one freeze-thaw cycle than the latter and repair this damage more slowly. Exposure of plateau-phase cells to freeze-thaw damage lengthens the time required to traverse the cell cycle in the exposed generation. These cells may more closely represent the state in tissues than exponential-phase populations.
处于平台期的未受保护的哺乳动物细胞对冻融损伤的敏感性至少是指数生长期细胞的四倍。在经历一次冻融循环后，前者遭受的亚致死损伤比后者多约15% - 20%，且修复这种损伤的速度更慢。将平台期细胞暴露于冻融损伤会延长受暴露代细胞穿越细胞周期所需的时间。与指数生长期群体相比，这些细胞可能更接近组织中的状态。
