Treutiger C J, Carlson J, Scholander C, Wahlgren M
Microbiology and Tumor Biology Centre, Karolinska Institutet, Stockholm, Sweden.
Am J Trop Med Hyg. 1998 Aug;59(2):202-7. doi: 10.4269/ajtmh.1998.59.202.
We describe morphologic characteristics of acridine orange-stained Plasmodium falciparum-infected erythrocytes and the sequential expression of several adhesion phenomena. In particular, we have studied when the adhesive and antigenic modifications appear on the infected erythrocyte surface that mediate binding to C32 melanoma cells (cytoadherence) or to erythrocytes (rosette formation) during a complete 48-hr life cycle of the parasite. The C32 melanoma cell binding started at about 12 hr and was seen during the whole life cycle with a peak around 28 hr (650 infected erythrocytes/100 C32 melanoma cells). Rosettes started to appear and immunoglobulin was found bound to the parasitized red blood cell (PRBC) somewhat later (16-20 hr). These adhesive events culminated at the mid-trophozoite/schizont stage (24-36 hr) with rosette formation and an immunoglobulin binding rate of about 50%, which decreased to about half of the peak values at the end of the life cycle. Serum-induced agglutination of the infected erythrocytes was also most extensive at 24-36 hr, but agglutination was seen with all late stage parasites, i.e., both trophozoites and schizonts at 24-48 hr of age. Taken together, adhesion to C32 melanoma cells starts prior to that of rosette formation, immunoglobulin binding, or serum-induced agglutination.
我们描述了吖啶橙染色的恶性疟原虫感染红细胞的形态学特征以及几种黏附现象的顺序表达。特别是，我们研究了在寄生虫完整的48小时生命周期中，介导与C32黑色素瘤细胞结合（细胞黏附）或与红细胞结合（玫瑰花结形成）的黏附性和抗原性修饰何时出现在感染红细胞表面。与C32黑色素瘤细胞的结合在约12小时开始，并在整个生命周期中可见，在约28小时达到峰值（650个感染红细胞/100个C32黑色素瘤细胞）。玫瑰花结开始出现以及发现免疫球蛋白结合到被寄生的红细胞（PRBC）的时间稍晚（16 - 20小时）。这些黏附事件在滋养体/裂殖体中期（24 - 36小时）达到顶峰，玫瑰花结形成以及免疫球蛋白结合率约为50%，在生命周期结束时降至峰值的约一半。感染红细胞的血清诱导凝集在24 - 36小时也最为广泛，但在所有晚期寄生虫中都可见凝集，即24 - 48小时龄的滋养体和裂殖体。综上所述，与C32黑色素瘤细胞的黏附先于玫瑰花结形成、免疫球蛋白结合或血清诱导凝集。
