Kandiah D A, Krilis S A
Department of Immunology, Allergy and Infectious Disease, University of New South Wales, The St. George Hospital, Kogarah, Australia.
Thromb Haemost. 1998 Aug;80(2):250-7.
"Antiphospholipid" (aPL) antibodies comprise two main groups of antibodies, lupus anticoagulant (LA) antibodies and "anticardiolipin" (aCL) antibodies which can be separated by certain chromatographic techniques. In this study we analysed the plasma of 10 patients with aPL antibodies, and were able to demonstrate that in four patients with both clotting test reactivities, the dilute Russell's Viper Venom Time (dRVVT) activity can be separated from the dilute Kaolin Clotting Time (dKCT) reactivity by using a polyacrylamide-immobilised phosphatidylserine column but not with phospholipid liposomes. The differential reactivity of the autoantibodies in this patient population is not due to binding to beta2GPI, prothrombin or protein C in solid-phase immunoassays. Hence LA antibodies detected in different phospholipid-dependent clotting tests detect different populations of antibodies in some APS patients and the routine detection of LA antibodies should be performed with at least two clotting tests looking at different coagulation reactions.
“抗磷脂”（aPL）抗体包括两大类抗体，即狼疮抗凝物（LA）抗体和“抗心磷脂”（aCL）抗体，它们可通过某些色谱技术分离。在本研究中，我们分析了10例aPL抗体患者的血浆，并能够证明，在4例同时具有凝血试验反应性的患者中，使用聚丙烯酰胺固定化磷脂酰丝氨酸柱而非磷脂脂质体，可将稀释的罗素蝰蛇毒时间（dRVVT）活性与稀释的高岭土凝血时间（dKCT）反应性分离。该患者群体中自身抗体的差异反应性并非由于在固相免疫测定中与β2糖蛋白I、凝血酶原或蛋白C结合。因此，在不同的磷脂依赖性凝血试验中检测到的LA抗体在一些抗磷脂综合征（APS）患者中检测到不同群体的抗体，并且LA抗体的常规检测应至少通过两种观察不同凝血反应的凝血试验来进行。
