Kandiah D A, Krilis S A
Department of Immunology, Allergy and Infectious Disease, University of New South Wales, St George Hospital, Kogarah, Australia.
Lupus. 1998;7(5):323-32. doi: 10.1191/096120398678920253.
Lupus anticoagulant (LA) antibodies have been shown to be directed to protein-phospholipid complexes. In this study, we report on LA antibodies from patients with the 'antiphospholipid' syndrome (APS), that are directed to prothrombin and beta2-glycoprotein I, but not to the complexes of these plasma proteins to anionic phospholipids. The anti-prothrombin antibodies studied had different reactivities in two clotting assays: the dilute Russell's viper venom time (dRVVT) and the dilute kaolin clotting time (dKCT). Anti-prothrombin and anti-beta2-glycoprotein I (anti-beta2GPI) antibodies, affinity-purified from one patient with APS were not cross-reactive and had different effects in the dRVVT and dKCT clotting tests. Polyclonal anti-prothrombin antibodies, affinity-purified on a prothrombin column, from two patients with prothrombin reactivity in their plasma, have affinity constants to prothrombin of 104 and 192 nM. The patient with affinity-purified antibodies to prothrombin and beta2GPI, had affinity constants to prothrombin and beta2GPI, respectively, of 192 nM and 3030 nM, respectively. LA antibodies are a heterogeneous population of antibodies that have different immunological specificities and clotting test reactivities in different patients.
狼疮抗凝物（LA）抗体已被证明针对蛋白质 - 磷脂复合物。在本研究中，我们报告了来自“抗磷脂”综合征（APS）患者的LA抗体，这些抗体针对凝血酶原和β2 - 糖蛋白I，但不针对这些血浆蛋白与阴离子磷脂的复合物。所研究的抗凝血酶原抗体在两种凝血试验中具有不同的反应性：稀释蝰蛇毒时间（dRVVT）和稀释高岭土凝血时间（dKCT）。从一名APS患者中亲和纯化的抗凝血酶原抗体和抗β2 - 糖蛋白I（抗β2GPI）抗体没有交叉反应性，并且在dRVVT和dKCT凝血试验中具有不同的作用。从两名血浆中具有凝血酶原反应性的患者中，在凝血酶原柱上亲和纯化的多克隆抗凝血酶原抗体，对凝血酶原的亲和常数分别为104和192 nM。具有亲和纯化的抗凝血酶原和β2GPI抗体的患者，对凝血酶原和β2GPI的亲和常数分别为192 nM和3030 nM。LA抗体是一类异质性抗体，在不同患者中具有不同的免疫特异性和凝血试验反应性。
