Breyer U, Villumsen K
Eur J Clin Pharmacol. 1976 Mar 22;09(5-6):457-65. doi: 10.1007/BF00606565.
A method has been developed for the determination of perazine, clozapine, imipramine and amitriptyline and their demethylated metabolites in plasma. Other metabolites measured were perazine sulfoxide and the N-oxides of clozapine and perazine, the latter two following their reduction to the parent drugs with ascorbic acid. 10-Hydroxynortriptyline was identified as an amitriptyline metabolite in plasma. The general procedure included extraction of alkalinized plasma samples (3 - 6 g) with benzene or toluene and thin layer chromatography of the extracts, followed by reflectance photometry of the plates at appropriate wave lengths in ultraviolet light. Spots of questionable identity were further characterized by two-dimensional chromatography and by colour reactions. Therecoveries of compounds added in therapeutic concentrations were between 70 and 98 %. The limits of detectability were 5 - 10 ng/g plasma.
已开发出一种用于测定血浆中奋乃静、氯氮平、丙咪嗪和阿米替林及其去甲基代谢物的方法。所测定的其他代谢物有奋乃静亚砜以及氯氮平和奋乃静的N - 氧化物，后两者在用抗坏血酸还原为母体药物后进行测定。10 - 羟基去甲替林被鉴定为血浆中阿米替林的一种代谢物。一般程序包括用苯或甲苯萃取碱化的血浆样品（3 - 6克），对萃取物进行薄层色谱分析，然后在紫外光下于适当波长对薄板进行反射光度测定。通过二维色谱分析和颜色反应进一步鉴定身份存疑的斑点。治疗浓度下添加化合物的回收率在70%至98%之间。可检测限为5 - 10纳克/克血浆。
