对抑郁症患者血浆去甲替林的一种简化放射免疫测定法与高压液相色谱法和气液色谱法的比较。
A simplified radioimmunoassay of plasma nortriptyline in depressed patients compared with high-pressure liquid chromatography and gas-liquid chromatography.
作者信息
Sayegh J F
出版信息
Neurochem Res. 1986 Feb;11(2):193-206. doi: 10.1007/BF00967968.
Antiserum to nortriptyline was generated in male New Zealand rabbits innoculated with n-succinylnortriptyline-bovine serum albumin conjugates. The antiserum was used at a final dilution of 1:4000 and tritiated imipramine was used as the label antigen. An accurate, sensitive, and specific radioimmunoassay of depressed patients' plasma or serum nortriptyline is described. The accuracy was good with a recovery range of 90-100% with a mean of 94%. The method can be used to measure nortriptyline concentration in the range of 0.1 microgram/liter to 100 micrograms/liter without prior extraction and purification of plasma or serum. Results of this method correlate well with those obtained by high-pressure liquid chromatography (r = 0.979) and by gas-liquid chromatography (r = 0.98). The specificity of the antiserum was examined by studying the cross-reactivity of 20 different psychopharmacological compounds, including nortriptyline's metabolites.
用n - 琥珀酰去甲替林 - 牛血清白蛋白偶联物免疫雄性新西兰兔，制备去甲替林抗血清。抗血清的终浓度为1:4000，用氚标记的丙咪嗪作为标记抗原。本文描述了一种准确、灵敏且特异的放射免疫分析法，用于检测抑郁症患者血浆或血清中的去甲替林。该方法准确性良好，回收率范围为90 - 100%，平均回收率为94%。该方法可用于测量血浆或血清中浓度在0.1微克/升至100微克/升范围内的去甲替林，无需事先对血浆或血清进行提取和纯化。该方法的结果与通过高压液相色谱法（r = 0.979）和气相色谱法（r = 0.98）获得的结果高度相关。通过研究包括去甲替林代谢物在内的20种不同精神药理化合物的交叉反应性，对抗血清的特异性进行了检测。
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