使用宫腔镜导管对输卵管进行插管后的超微结构评估。
Ultrastructural evaluation following catheterization of the fallopian tube with a hysteroscopic catheter.
作者信息
Kitamura S, Miyazaki T, Iwata S, Akaboshi K, Osawa Y, Yoshimura Y
机构信息
Department of Obstetrics and Gynecology, School of Medicine, Keio University, Tokyo, Japan.
出版信息
J Assist Reprod Genet. 1998 Aug;15(7):411-7. doi: 10.1007/BF02744933.
PURPOSE
Our purpose was to assess the morphology and ultrastructural changes in the tubal epithelium following catheterization of the fallopian tube.
METHODS
Fallopian tubes were obtained from 20 women who had undergone hysterectomies. Catheterization was performed in 20 tubes using a catheter developed for hysteroscopic tubal embryo transfer. The catheter has a 3-French diameter, tapering to 2 French (0.66 mm) at the tip portion. The 20 contralateral tubes served as controls and were not catheterized. Ultrastructural changes were examined by scanning electron microscopy and transmission electron microscopy.
RESULTS
Scanning electron microscopy showed no transformation or defects of the tubal epithelium surface in catheterized or control tubes. Transmission electron microscopy showed no significant differences in the percentage of abnormal desmosomes and the percentage of basement membrane in ciliated and nonciliated cells between catheterized and noncatheterized tubes. No transformation or defects were observed in catheterized or noncatheterized tubes.
CONCLUSIONS
These findings suggest that catheterization of the tube using a hysteroscopic catheter caused no acute damage to the tubal epithelium.
目的
我们的目的是评估输卵管插管后输卵管上皮的形态和超微结构变化。
方法
从20例接受子宫切除术的女性获取输卵管。使用专为宫腔镜输卵管胚胎移植开发的导管对20条输卵管进行插管。该导管直径为3法式，尖端部分逐渐变细至2法式（0.66毫米）。20条对侧输卵管作为对照，未进行插管。通过扫描电子显微镜和透射电子显微镜检查超微结构变化。
结果
扫描电子显微镜显示，插管或对照输卵管的输卵管上皮表面无转化或缺陷。透射电子显微镜显示，插管和未插管输卵管的纤毛细胞和非纤毛细胞中异常桥粒百分比和基底膜百分比无显著差异。插管或未插管输卵管均未观察到转化或缺陷。
结论
这些发现表明，使用宫腔镜导管对输卵管进行插管不会对输卵管上皮造成急性损伤。
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