Wood P, Caldwell D E, Evans E, Jones M, Korber D R, Wolfhaardt G M, Wilson M, Gilbert P
Department of Pharmacy, University of Manchester, UK.
J Appl Microbiol. 1998 Jun;84(6):1092-8. doi: 10.1046/j.1365-2672.1998.00446.x.
Transition metal catalysts were incorporated into polymers which formed the surface for bacterial attachment and biofilm formation in a constant depth film fermenter (100 microns thickness), flow chamber (about 30 microns thickness) and in batch culture (< 30 microns thickness). The catalysts drive the breakdown of persulphates to reactive oxygen species. When Pseudomonas aeruginosa biofilms were exposed to dilute solutions of potassium monopersulphate (20 micrograms ml-1-1 mg ml-1), significant enhancement of killing was notable for catalyst-containing surfaces over that of controls. The degree of enhancement was greatest for thin films, but was nevertheless significant for the 100 microns thick biofilms. Fluorescence probes and viability staining, in conjunction with laser confocal microscopy, showed that reactive species were generated at the biofilm-substratum interface and killed the biofilm from the inside. Reaction-diffusion limitation now concentrates the active species within the biofilm rather than protecting it, and a diffusion bump is established whereby further treatment agent is drawn to the substratum enabling relatively thick biofilms to be disinfected.
过渡金属催化剂被掺入聚合物中，这些聚合物在恒深膜发酵罐（100微米厚）、流动腔室（约30微米厚）和分批培养（<30微米厚）中形成细菌附着和生物膜形成的表面。催化剂促使过硫酸盐分解为活性氧。当铜绿假单胞菌生物膜暴露于单过硫酸钾稀溶液（20微克/毫升 - 1毫克/毫升）时，与对照相比，含催化剂表面的杀菌效果显著增强。薄膜的增强程度最大，但对于100微米厚的生物膜而言也很显著。荧光探针和活力染色结合激光共聚焦显微镜显示，活性物质在生物膜 - 基质界面产生，并从内部杀死生物膜。反应 - 扩散限制现在将活性物质集中在生物膜内而不是保护它，并且形成了一个扩散凸起，从而使更多的处理剂被吸引到基质上，使相对较厚的生物膜能够被消毒。
