Dietlein T S, Jacobi P C, Frie C, Paulsson M, Krieglstein G K
Department of Ophthalmology, University of Cologne, Germany.
Graefes Arch Clin Exp Ophthalmol. 1998 Aug;236(8):597-601. doi: 10.1007/s004170050128.
An abnormally increased presence of type VI collagen has been shown in the lamina cribrosa of patients with primary and secondary glaucoma. This study was undertaken to investigate the pattern of type VI collagen within the aqueous outflow structures of glaucoma patients.
Trabecular meshwork samples of eight normal donor eyes and trabeculectomy specimens of 21 patients with different types of glaucoma were processed for either cryo-sectioning or for paraffin embedding. Immunohistochemical staining was conducted by use of polyclonal rabbit antibodies against human collagen type VI.
Immunoreactivity for type VI collagen was evident in the cores of all trabecular beams. The strongest staining was detected in the uveal region of the human trabecular meshwork. Immunohistochemical labelling for type VI collagen was not more pronounced in the aqueous outflow structures of glaucoma patients than in normal eyes.
Collagen type VI is a ubiquitous structural component of the extracellular matrix in the human trabecular meshwork. However, type VI collagen does not appear to be of greater importance for the increased trabecular outflow resistance in glaucoma patients than in normal eyes.
原发性和继发性青光眼患者的筛板中已显示出VI型胶原蛋白异常增多。本研究旨在调查青光眼患者房水流出结构中VI型胶原蛋白的分布模式。
对8只正常供体眼的小梁网样本和21例不同类型青光眼患者的小梁切除术标本进行冷冻切片或石蜡包埋处理。使用抗人VI型胶原蛋白的多克隆兔抗体进行免疫组织化学染色。
在所有小梁束的核心部位均可见VI型胶原蛋白的免疫反应性。在人小梁网的葡萄膜区域检测到最强的染色。VI型胶原蛋白的免疫组织化学标记在青光眼患者的房水流出结构中并不比正常眼睛更明显。
VI型胶原蛋白是人类小梁网细胞外基质中普遍存在的结构成分。然而，VI型胶原蛋白对于青光眼患者小梁网流出阻力增加的重要性似乎并不比正常眼睛更大。
