使用填充有全甲基-β-环糊精固定相的毛细管通过压力辅助电色谱法进行对映体分离。
Enantiomer separation by pressure-supported electrochromatography using capillaries packed with a permethyl-beta-cyclodextrin stationary.
作者信息
Wistuba D, Czesla H, Roeder M, Schurig V
机构信息
Institute of Organic Chemistry, University of Tübingen, Germany.
出版信息
J Chromatogr A. 1998 Jul 31;815(2):183-8. doi: 10.1016/s0021-9673(98)00472-5.
Efficient enantiomer separation by pressure-assisted, micro-packed capillary electrochromatography (CEC) has been carried out using a permethyl-beta-cyclodextrin-modified silica support (PM-beta-CD-silica). When comparing this method with micro-packed-high-performance liquid chromatography in the single-column-mode, CEC displays higher column efficiencies (about three times higher theoretical plate numbers at comparable elution times). The pressure support (about 10 bar), applied to avoid bubble formation, has a negligible influence on elution times in CEC. The influence of the type and composition of organic modifiers is described.
采用全甲基化β-环糊精修饰硅胶载体（PM-β-CD-硅胶），通过压力辅助微填充毛细管电色谱法（CEC）实现了对映体的高效分离。在单柱模式下将该方法与微填充高效液相色谱法进行比较时，CEC显示出更高的柱效（在可比洗脱时间下理论塔板数约高三倍）。为避免气泡形成而施加的压力支持（约10巴）对CEC中的洗脱时间影响可忽略不计。文中描述了有机改性剂的类型和组成的影响。
Zotero 插件