Magnusson U, Greko C
Department of Obstetrics and gynaecology, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Zentralbl Veterinarmed B. 1998 Aug;45(6):353-61. doi: 10.1111/j.1439-0450.1998.tb00804.x.
The objective of this study was to determine whether phagocytic capacity and opsonic activity in blood and mammary secretions of sows are impaired at parturition compared with later on during lactation. The study comprised eight primiparous sows (Landrace x Yorkshire) free from clinical signs of disease. Blood and mammary secretion samples were collected within 48 h of parturition and 7 and 16 days after parturition. Numbers and proportion of polymorphonuclear neutrophils (PMN) were determined in blood and mammary secretions. Phagocytic capacity was assessed in whole blood and in a cell suspension derived from mammary secretions. Opsonic activity was assessed in serum and i cell-depleted, skimmed mammary secretions. The two assays were based on chemiluminescence, both having zymosan and Escherichia coli as target particles. Numbers and proportion of PMN in mammary secretions were higher (P < 0.05) at parturition than later on during lactation. A parturition, phagocytic capacity in cell suspensions derived from mammary secretions was higher for both (P < 0.05) and E. coli (P < 0.1). However, when phagocytic capacity was related to the number of PMN in the suspension no such difference was observed. The opsonic activity in cell-depleted, skimmed mammary secretions at parturition was lower (P < 0.05) for zymosan but not for E. coli. None of the described variations were reflected in blood or serum. The findings of this study do not unequivocally support the theory that an immune suppression at parturition in the sow can help explain the increased incidence of coliform mastitis at that time.
本研究的目的是确定与泌乳后期相比，母猪分娩时血液和乳腺分泌物中的吞噬能力及调理活性是否受损。该研究包括八头无疾病临床症状的初产母猪（长白猪×约克夏猪）。在分娩后48小时内以及分娩后7天和16天采集血液和乳腺分泌物样本。测定血液和乳腺分泌物中多形核中性粒细胞（PMN）的数量和比例。在全血和源自乳腺分泌物的细胞悬液中评估吞噬能力。在血清和细胞耗尽的脱脂乳腺分泌物中评估调理活性。这两种测定均基于化学发光，均以酵母聚糖和大肠杆菌作为靶颗粒。乳腺分泌物中PMN的数量和比例在分娩时高于泌乳后期（P<0.05）。分娩时，源自乳腺分泌物的细胞悬液对酵母聚糖（P<0.05）和大肠杆菌（P<0.1）的吞噬能力均较高。然而，当吞噬能力与悬液中PMN的数量相关时，未观察到此类差异。分娩时细胞耗尽的脱脂乳腺分泌物中对酵母聚糖的调理活性较低（P<0.05），但对大肠杆菌则不然。所描述的这些变化均未在血液或血清中体现。本研究结果并未明确支持母猪分娩时免疫抑制有助于解释此时大肠埃希菌性乳腺炎发病率增加这一理论。
