Kaiser C, Ferro-Novick S
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.
Curr Opin Cell Biol. 1998 Aug;10(4):477-82. doi: 10.1016/s0955-0674(98)80062-8.
Two crucial aspects of transport vesicle function have recently been reconstituted using purified proteins and chemically defined lipid bilayers. The reconstituted steps are the assembly of a polymeric protein coat on the cytosolic surface of the membrane, and bilayer fusion based on the pairing of proteins in the vesicle and target membrane. These advances now set the stage to address major unresolved questions of how vesicle budding and vesicle fusion are regulated, how specific cargo molecules are incorporated into vesicles, and how vesicles find their target membrane.
最近，利用纯化的蛋白质和化学定义的脂质双层，重新构建了运输囊泡功能的两个关键方面。重新构建的步骤包括在膜的胞质表面组装聚合蛋白衣被，以及基于囊泡和靶膜中蛋白质配对的双层融合。这些进展为解决关于囊泡出芽和囊泡融合如何被调控、特定货物分子如何被纳入囊泡以及囊泡如何找到其靶膜等主要未解决问题奠定了基础。
