Sutton M D, Holmes N G, Brennan F B, Binns M M, Kelly E P, Duke E J
Zoology Department, University College Dublin, Belfield, Republic of Ireland.
Anim Genet. 1998 Jun;29(3):168-72. doi: 10.1046/j.1365-2052.1998.00286.x.
Pairwise analysis of HinfI/33.6 DNA fingerprints from a total of one hundred and fifty-three Irish greyhounds of known pedigree were used to determine band-share estimates of unrelated, first-degree and second-degree relationships. Forty-eight unrelated Irish greyhounds were used to determine allele frequencies for three single-locus minisatellites, and following a preliminary screen, eight of the most polymorphic tetra-nucleotide microsatellites from a panel of 15. The results indicated that both band-share estimates by DNA fingerprinting and microsatellite allele frequencies are highly effective in resolving parentage in this greyhound population, while single-locus minisatellites showed limited polymorphism and could not be used alone for routine parentage testing in this breed. The present study also demonstrated that, to obtain optimal resolution of parentage, sample sets of known pedigree status are required to determine the band-share distribution and/or microsatellite allele frequencies.
对总共153只已知谱系的爱尔兰灵缇犬的HinfI/33.6 DNA指纹进行成对分析，以确定无亲缘关系、一级亲缘关系和二级亲缘关系的条带共享估计值。使用48只无亲缘关系的爱尔兰灵缇犬来确定三个单基因座微卫星的等位基因频率，经过初步筛选后，从15个微卫星组成的面板中选取了8个多态性最高的四核苷酸微卫星。结果表明，通过DNA指纹分析得到的条带共享估计值和微卫星等位基因频率在解决该灵缇犬群体的亲子关系方面都非常有效，而单基因座微卫星显示出有限的多态性，不能单独用于该品种的常规亲子关系检测。本研究还表明，为了获得最佳的亲子关系分辨率，需要已知谱系状态的样本集来确定条带共享分布和/或微卫星等位基因频率。
