Shirai N, Momma K, Ozawa S, Hashimoto W, Kito M, Utsumi S, Murata K
Research Institute for Food Science, Kyoto University, Japan.
Biosci Biotechnol Biochem. 1998 Jul;62(7):1461-4. doi: 10.1271/bbb.62.1461.
A detection technique for the genetically engineered food, glyphosate-tolerant soybean (GTS), was designed. Commercial soybeans imported from North America were cultured in pots and genomic DNA was isolated from their leaves. To detect the genes, promoter and terminator, involved in the expression of glyphosate tolerance, PCR was done using the genomic DNA and chemically synthesized primers specific to the genes. DNAs with predicted sizes were amplified and confirmed by DNA sequencing to be the genes responsible for the expression of glyphosate tolerance. Glyphosate-tolerant soybeans were found to form approximately 1.1% of the commercial soybeans, when commercially available soybeans were cultivated and number of soybeans resistant to glyphosate was found. This level is somewhat lower than an estimated value announced officially on the basis of the cultivation area of the glyphosate-tolerant soybeans.
设计了一种用于检测转基因食品——抗草甘膦大豆（GTS）的检测技术。从北美进口的商用大豆种植于花盆中，并从其叶片中分离基因组DNA。为了检测与抗草甘膦表达相关的基因、启动子和终止子，使用基因组DNA和针对这些基因的化学合成引物进行PCR。扩增出具有预测大小的DNA，并通过DNA测序确认其为负责抗草甘膦表达的基因。当种植市售大豆并统计抗草甘膦大豆数量时，发现抗草甘膦大豆约占商用大豆的1.1%。这一水平略低于根据抗草甘膦大豆种植面积官方公布的估计值。
