Blahová J, Lesická-Hupková M, Králiková K, Krcméry V, Krcméryová T, Kubonová K
Institute of Preventive and Clinical Medicine, Bratislava, Slovak Republic.
J Chemother. 1998 Aug;10(4):291-4. doi: 10.1179/joc.1998.10.4.291.
We describe the further occurrence of Salmonella enteritidis producing an extended spectrum beta-lactamase (ESBL) and transferring cefotaxime, ceftazidime and aztreonam resistance. In a previous communication we describe the isolation of a strain of S. enteritidis (No. 35) from a septic neonate with high-level resistance to cefuroxime, cefotaxime, ceftazidime and aztreonam. Six weeks later, a second neonate was found, in the same Pediatric University Hospital, to be infected with apparently the same strain of S. enteritidis (No. 100) which was isolated from blood with similar properties as the strain No. 35. In this strain we were able to study, in more detail, the transferability of its resistance to 3rd generation cephalosporins and its ESBL production. In this report we describe results of transfer experiments, relative rates of hydrolysis (Vmax) and ESBL tests which indicate that the capacity to produce ESBL was transferred from the multiply-resistant strain of S. enteritidis No. 100 to S. typhimurium LT-2 rif+str+.
我们描述了肠炎沙门氏菌产生超广谱β-内酰胺酶（ESBL）并传递头孢噻肟、头孢他啶和氨曲南耐药性的进一步情况。在之前的一篇通讯中，我们描述了从一名对头孢呋辛、头孢噻肟、头孢他啶和氨曲南具有高水平耐药性的败血症新生儿中分离出一株肠炎沙门氏菌（编号35）。六周后，在同一所儿科大学医院发现第二名新生儿感染了明显相同的肠炎沙门氏菌菌株（编号100），该菌株从血液中分离出来，其特性与35号菌株相似。在该菌株中，我们能够更详细地研究其对第三代头孢菌素耐药性的转移性及其ESBL的产生情况。在本报告中，我们描述了转移实验结果、相对水解速率（Vmax）和ESBL测试，这些结果表明产生ESBL的能力从多重耐药的肠炎沙门氏菌100号菌株转移到了鼠伤寒沙门氏菌LT-2 rif+str+。
