Medina S, Del Río M, Manuel Victor V, Hernánz A, De la Fuente M
Department of Animal Physiology, Faculty of Biological Science, Complutense University, Madrid, Spain.
Mech Ageing Dev. 1998 May 15;102(2-3):249-61. doi: 10.1016/s0047-6374(98)00014-1.
The general immunodepression found in ageing organisms may be related to changes in the neuroimmune network. In the present study, the migration capacity of lymphocytes from BALB/c mice of three different ages: young (12 +/- 2 weeks), adult (24 +/- 2 weeks) and old (72 +/- 2 weeks), has been assayed in vitro in response to three neuropeptides: sulfated cholecystokinin octapeptide (CCK-8s), gastrin-releasing peptide (GRP) and neuropeptide Y (NPY) in a physiological range of concentrations (10(-8)-10(-12) M). The capacity of migration to a chemical gradient or chemotaxis was studied by the Boyden's technique using f-met-leu-phe at 10(-8) M as chemoattractant. The results show a different response of lymphocytes to the different neuropeptides, as wells as to age, concentrations and locations studied. However, some similarities were found, for instance the three neuropeptides inhibited chemotaxis in thymus. The stimulatory effects that GRP and NPY exerted in young and adult mice were not observed in old animals. CCK-8s inhibited the chemotaxis in every organ studied, with the effect being more striking in old mice. Our conclusion is that stimulatory effects of the neuropeptides disappear or become inhibitory with ageing.
在衰老生物体中发现的一般免疫抑制可能与神经免疫网络的变化有关。在本研究中，已在体外测定了来自三种不同年龄（年轻（12±2周）、成年（24±2周）和老年（72±2周））的BALB/c小鼠淋巴细胞对三种神经肽（硫酸化八肽胆囊收缩素（CCK-8s）、胃泌素释放肽（GRP）和神经肽Y（NPY））在生理浓度范围（10⁻⁸ - 10⁻¹² M）下的迁移能力。使用10⁻⁸ M的f-met-leu-phe作为趋化剂，通过博伊登技术研究了向化学梯度迁移的能力或趋化性。结果显示淋巴细胞对不同神经肽以及所研究的年龄、浓度和部位有不同反应。然而，也发现了一些相似之处，例如这三种神经肽在胸腺中均抑制趋化性。在老年动物中未观察到GRP和NPY在年轻和成年小鼠中所发挥的刺激作用。CCK-8s在每个研究器官中均抑制趋化性，在老年小鼠中这种作用更为显著。我们的结论是，随着衰老，神经肽的刺激作用消失或变为抑制作用。
