Medina S, Del Río M, Hernanz A, De la Fuente M
Department of Animal Physiology, Faculty of Biological Sciences, Complutense University, Av. Complutense s/n, E-28040, Madrid, Spain.
Regul Pept. 2000 Nov 24;95(1-3):35-45. doi: 10.1016/s0167-0115(00)00134-8.
The two-way communication between the nervous and immune system is currently well-known, but the age-related changes in this communication have been scarcely studied. In the present work, we have investigated the in vitro effects of neuropeptide Y (NPY) at concentrations ranging from 10(-13) to 10(-7) M on the adherence and chemotaxis capacities of spleen, axillary node, thymus and peritoneum leukocytes from BALB/c mice. The NPY effect on these functions was examined on cells from animals of four different ages, i.e. young (12+/-2 weeks old), adult (24+/-2 weeks old), mature (50+/-2 weeks old) and old (72+/-2 weeks old). In young animals, NPY stimulates the adherence of leukocytes from spleen, axillary nodes and thymus and inhibits it in cells from peritoneum. In adult animals NPY inhibits the adherence of leukocytes from thymus. These effects disappear with ageing in all locations. Chemotaxis is stimulated by this neuropeptide at all ages in cells from axillary nodes and peritoneum, but this effect is absent in old mice. NPY exerts an inhibitory effect on the chemotaxis of leukocytes from thymus at all ages studied. These NPY effects on leukocytes seem to be carried out through adherent cells.
神经与免疫系统之间的双向通讯目前已广为人知，但这种通讯中与年龄相关的变化却鲜有研究。在本研究中，我们研究了浓度范围为10(-13)至10(-7)M的神经肽Y（NPY）对BALB/c小鼠脾脏、腋窝淋巴结、胸腺和腹膜白细胞黏附及趋化能力的体外影响。在来自四个不同年龄组动物（即幼年（12±2周龄）、成年（24±2周龄）、成熟（50±2周龄）和老年（72±2周龄））的细胞上检测了NPY对这些功能的影响。在幼年动物中，NPY刺激脾脏、腋窝淋巴结和胸腺白细胞的黏附，而抑制腹膜细胞的黏附。在成年动物中，NPY抑制胸腺白细胞的黏附。所有部位的这些作用都会随着衰老而消失。在腋窝淋巴结和腹膜细胞中，该神经肽在所有年龄段均刺激趋化作用，但老年小鼠中不存在这种作用。在所研究的所有年龄段，NPY对胸腺白细胞的趋化作用均有抑制作用。NPY对白细胞的这些作用似乎是通过黏附细胞实现的。
