Cruise G M, Scharp D S, Hubbell J A
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena 91125, USA.
Biomaterials. 1998 Jul;19(14):1287-94. doi: 10.1016/s0142-9612(98)00025-8.
Hydrogel membranes formed by interfacially photopolymerizing poly(ethylene glycol) (PEG) diacrylate precursor solution were prepared from PEG diacrylate of molecular weights (MW) ranging from 2000 (2K) to 20000 (20K) with concentrations ranging from 10% to 30% w/w. The effects of PEG diacrylate MW and concentration in the membrane precursor solution upon the diffusivities of vitamin B12, myoglobin, ovalbumin, albumin, and IgG were determined. Regardless of the concentration of the PEG diacrylate in the precursor solution, hydrogels prepared with PEG 2K, 4K, and 8K diacrylate were impermeable to proteins with a size equal to or larger than myoglobin (22 kDa), while hydrogels prepared with PEG 20K diacrylate were impermeable to proteins with a size equal to or larger than ovalbumin (45 kDa). Similarities between hydrogels formed from PEG 2K, 4K, and 8K diacrylates were also seen in calculations of the molecular weight between crosslinks and the mesh size, with values in the range of 150-750 g/mol and 15-35 A, respectively, depending on PEG diacrylate concentration. In contrast, hydrogels formed from PEG 20K diacrylate had molecular weight between crosslinks ranging from 1150 to 2000 g/mol and mesh sizes ranging from 45-70 A, with larger values being observed in membranes polymerized from more dilute PEG diacrylate precursor.
通过界面光聚合聚乙二醇（PEG）二丙烯酸酯前体溶液形成的水凝胶膜，是由分子量（MW）范围为2000（2K）至20000（20K）、浓度范围为10%至30% w/w的PEG二丙烯酸酯制备而成。测定了膜前体溶液中PEG二丙烯酸酯的分子量和浓度对维生素B12、肌红蛋白、卵清蛋白、白蛋白和IgG扩散率的影响。无论前体溶液中PEG二丙烯酸酯的浓度如何，用PEG 2K、4K和8K二丙烯酸酯制备的水凝胶对尺寸等于或大于肌红蛋白（22 kDa）的蛋白质是不可渗透的，而用PEG 20K二丙烯酸酯制备的水凝胶对尺寸等于或大于卵清蛋白（45 kDa）的蛋白质是不可渗透的。在计算交联点之间的分子量和网孔尺寸时，也可以看到由PEG 2K、4K和8K二丙烯酸酯形成的水凝胶之间的相似性，其值分别在150 - 750 g/mol和15 - 35 Å范围内，具体取决于PEG二丙烯酸酯的浓度。相比之下，由PEG 20K二丙烯酸酯形成的水凝胶交联点之间的分子量范围为1150至2000 g/mol，网孔尺寸范围为45 - 70 Å，在由更稀的PEG二丙烯酸酯前体聚合而成的膜中观察到更大的值。
