Lahti R A, Cochrane E V, Roberts R C, Conley R R, Tamminga C A
Maryland Psychiatric Research Center, University of Maryland School of Medicine, Baltimore 21228, USA.
J Neural Transm (Vienna). 1998;105(4-5):507-16. doi: 10.1007/s007020050074.
[3H]Neurotensin binding and autoradiographic techniques were used to determine the distribution and density of neurotensin receptors in normal and schizophrenic postmortem brain tissue. Coronal hemi-brain blocks of tissue were cut at the level of the caudate and hippocampus from frozen brain tissue obtained from normal individuals with no known psychiatric or neurologic illnesses and from schizophrenic subjects off- or on-antipsychotic drugs at the time of death. Each hemi-block was further divided, sectioned, thaw mounted on to slides, incubated with [3H]neurotensin and apposed to film. Digitized images were analyzed for binding densities. Areas of intense binding include the substantia nigra, the entorhinal cortex, superficial layers of the cingulate, middle frontal, and insular cortices; and with moderate binding in nucleus accumbens, and caudate. Schizophrenic patients off- (3 months or more) or on-antipsychotic drugs at the time of death were tested; all patients showed a reduced level of neurotensin receptors in the caudate (68% of normals), cingulate (34%) and prefrontal cortices (25%).
采用[3H]神经降压素结合及放射自显影技术，测定正常和精神分裂症死后脑组织中神经降压素受体的分布及密度。从无已知精神或神经疾病的正常个体以及死亡时停用或正在服用抗精神病药物的精神分裂症患者获取的冷冻脑组织，在尾状核和海马水平切取冠状半脑块组织。每个半脑块进一步分割、切片、解冻后贴附于载玻片上，与[3H]神经降压素孵育并与胶片贴合。对数字化图像进行结合密度分析。结合强烈的区域包括黑质、内嗅皮质、扣带回、额中回和岛叶皮质的表层；伏隔核和尾状核结合中等。对死亡时停用（3个月或更长时间）或正在服用抗精神病药物的精神分裂症患者进行了检测；所有患者尾状核（正常水平的68%）、扣带回（34%）和前额叶皮质（25%）的神经降压素受体水平均降低。
