Albandar J M, Kingman A, Lamster I B
National Institute of Dental Research, Bethesda, Maryland, USA.
J Clin Periodontol. 1998 Aug;25(8):630-9. doi: 10.1111/j.1600-051x.1998.tb02499.x.
Analysis of beta-glucuronidase (betaG) in the gingival crevicular fluid (GCF) provides an indication of neutrophil influx into the crevicular environment. The aim of this study was to test the hypotheses that: (1) betaG is significantly elevated in individuals with early-onset periodontitis (EOP) and that betaG activity correlates with disease severity; and (2) betaG level may reflect the local bacterial challenge in the gingival crevice. The study subjects consisted of a sub-sample of individuals examined in the National Survey of Oral Health of United States Children, which was undertaken during the 1986/87 school year. A total of 249 individuals were selected based on presence or absence of clinical attachment loss at baseline. The individuals were examined a second time 6 years later and the clinical attachment loss was assessed, and subgingival plaque and GCF were collected. The subjects were classified into 3 types of EOP and a control group. BetaG activity in the GCF and the levels of 7 putative micro-organisms in the pocket were assessed. The generalized EOP group had the highest betaG activity, followed by the localized and incidental EOP groups, and the controls, respectively. There was a significant increase in betaG activity with the increase in probing depth. Also, sites with bleeding on probing had a significantly higher betaG activity than sites without bleeding. However, the effect of gingival inflammation on betaG activity was more evident in the generalized and localized EOP groups. Sites harboring high levels of one or more of the micro-organisms tended to have high betaG activity. There were moderate differences between the organisms with respect to their effect on betaG activity, but sites with high numbers of Porphyromonas gingivalis, Prevotella intermedia, or Treponema denticola also had the highest betaG activity. The present findings suggest that betaG activity in GCF from patients with EOP can be of value in the early identification of individuals at higher risk of developing EOP The findings also suggest that host mechanisms leading to higher betaG activity in EOP represent systemic responses and are only partly related to the presence of local factors at the site-level.
分析龈沟液（GCF）中的β-葡萄糖醛酸酶（βG）可显示中性粒细胞向龈沟环境的流入情况。本研究的目的是检验以下假设：（1）早发性牙周炎（EOP）患者的βG显著升高，且βG活性与疾病严重程度相关；（2）βG水平可能反映龈沟内的局部细菌刺激。研究对象包括在1986/87学年进行的美国儿童口腔健康全国调查中接受检查的个体的一个子样本。根据基线时是否存在临床附着丧失，共选择了249名个体。6年后对这些个体进行了第二次检查，评估了临床附着丧失情况，并收集了龈下菌斑和GCF。受试者被分为3种类型的EOP和一个对照组。评估了GCF中的βG活性以及袋内7种假定微生物的水平。广泛性EOP组的βG活性最高，其次分别是局限性和偶发性EOP组以及对照组。随着探诊深度增加，βG活性显著升高。此外，探诊出血部位的βG活性显著高于未出血部位。然而，牙龈炎症对βG活性的影响在广泛性和局限性EOP组中更为明显。含有一种或多种高水平微生物的部位往往具有较高的βG活性。这些微生物对βG活性的影响存在适度差异，但牙龈卟啉单胞菌、中间普氏菌或具核梭杆菌数量较多的部位也具有最高的βG活性。目前的研究结果表明，EOP患者GCF中的βG活性在早期识别患EOP风险较高的个体方面可能具有价值。研究结果还表明，导致EOP中βG活性升高的宿主机制代表全身反应，仅部分与部位水平的局部因素存在有关。
