Yakob M, Meurman J H, Sorsa T, Söder B
Department of Dental Medicine, Division of Periodontology, Karolinska Institutet, Huddinge, Sweden.
Oral Dis. 2013 Oct;19(7):694-701. doi: 10.1111/odi.12057. Epub 2013 Jan 7.
The aim was to assess the association between the presence of site-specific subgingival micro-organisms and the levels of matrix metalloproteinases-8 and matrix metalloproteinases-9 (MMP-8 and MMP-9) in gingival crevicular fluid (GCF).
The patient group consisted of 56 subjects with periodontitis and the control group of 43 subjects without periodontitis. GCF samples from four test sites for each subject were collected. Polymerase chain reaction was used to detect the presence of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, and Treponema denticola. MMP-8 concentrations were analyzed by a time-resolved immunofluorometric assay, and MMP-9 levels were determined by enzyme-linked immunosorbent assay. Student's unpaired t-test, chi-square test, and Fisher's exact P-value were calculated.
The presence of T. denticola in the test sites was significantly higher in the patient group than in the control group. The presence of T. forsythia and T. denticola was associated with increased levels of MMP-8 in the test sites. Respectively, site-specific presence of T. denticola was associated with an increase in MMP-9 levels in three of the four test sites.
The presence of subgingival micro-organisms in GCF, particularly T. denticola, appeared to induce a host response with an increased release of MMP-8 and MMP-9 in the test sites.
评估特定部位龈下微生物的存在与龈沟液（GCF）中基质金属蛋白酶-8和基质金属蛋白酶-9（MMP-8和MMP-9）水平之间的关联。
患者组由56名牙周炎患者组成，对照组由43名无牙周炎的受试者组成。收集每个受试者四个测试部位的GCF样本。采用聚合酶链反应检测伴放线聚集杆菌、牙龈卟啉单胞菌、中间普氏菌、福赛坦纳菌和具核梭杆菌的存在情况。通过时间分辨免疫荧光分析法分析MMP-8浓度，采用酶联免疫吸附测定法测定MMP-9水平。计算学生氏非配对t检验、卡方检验和Fisher精确P值。
患者组测试部位具核梭杆菌的存在率显著高于对照组。福赛坦纳菌和具核梭杆菌的存在与测试部位MMP-8水平升高相关。具核梭杆菌在特定部位的存在分别与四个测试部位中的三个部位MMP-9水平升高相关。
GCF中龈下微生物的存在，尤其是具核梭杆菌，似乎会诱导宿主反应，导致测试部位MMP-8和MMP-9的释放增加。
