重新审视锤头状核酶的序列特异性；NHH规则。
Sequence specificity of the hammerhead ribozyme revisited; the NHH rule.
作者信息
Kore A R, Vaish N K, Kutzke U, Eckstein F
机构信息
Max-Planck-Institut für Experimentelle Medizin, Hermann-Rein-Strasse 3, D-37075 Göttingen, Germany.
出版信息
Nucleic Acids Res. 1998 Sep 15;26(18):4116-20. doi: 10.1093/nar/26.18.4116.
Abstract
The sequence specificity of hammerhead ribozyme cleavage has been re-evaluated with respect to the NUH rule. Contrary to previous reports it was found that substrates with GAC triplets were also cleaved. This was established in three different sequence contexts. The rate of cleavage under single turnover conditions was between 3 and 7% that of cleavage 3' of GUC. Specificity of cleavage of substrates containing a central A in the cleavable triplet can be described as NAH, where N can be any nucleotide and H any nucleotide but G. As cleavage 3' of NCH triplets has recently been described, the NUH rule can be reformulated to NHH.
摘要
针对锤头状核酶切割的序列特异性，已根据NUH规则进行了重新评估。与之前的报道相反，发现含有GAC三联体的底物也会被切割。这在三种不同的序列背景下得到了证实。单轮反应条件下的切割速率为GUC 3'端切割速率的3%至7%。可切割三联体中含有中心A的底物的切割特异性可描述为NAH，其中N可以是任何核苷酸，H可以是除G以外的任何核苷酸。由于最近已报道了NCH三联体3'端的切割，因此NUH规则可重新表述为NHH。
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本文引用的文献
1
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Nucleic Acids Res. 1998 May 15;26(10):2279-85. doi: 10.1093/nar/26.10.2279.
2
Structural variation induced by different nucleotides at the cleavage site of the hammerhead ribozyme.锤头状核酶切割位点处不同核苷酸诱导的结构变异。
Biochemistry. 1998 Mar 24;37(12):4034-44. doi: 10.1021/bi972493z.
3
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4
Searching for the ideal partner.寻找理想伴侣。
Nat Biotechnol. 1998 Jan;16(1):24. doi: 10.1038/nbt0198-24.
5
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Eur J Biochem. 1997 Apr 1;245(1):1-16. doi: 10.1111/j.1432-1033.1997.t01-3-00001.x.
6
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