Thomas W R, Holt P G, Keast D
Infect Immun. 1978 May;20(2):468-75. doi: 10.1128/iai.20.2.468-475.1978.
Phagocytic function was studied in mice chronically exposed to cigarette smoke, and the effects of in vitro exposure to cigarette smoke on macrophage activity were also assessed. Cultures of radiolabeled Pseudomonas aeruginosa were employed to investigate phagocyte activity in vivo and in vitro. Mice were exposed on weekdays to fresh cigarette smoke for periods up to 37 weeks and the bactericidal and clearance activity of their lungs was measured. Both pulmonary clearance and bactericidal activity was impaired. The clearance of intravenously injected bacteria from the blood of smoke-exposed mice occurred at the same rate as in control mice, but the accumulation of radiolabel by the liver was decreased. In addition, the rate of elimination of radiolabel from the liver was less than the controls. Macrophages exposed to cigarette smoke in vitro initially had a depressed phagocytic rate, but if phagocytosis over a prolonged period was measured it was eventually enhanced over the rate of control macrophages. The vapor phase of cigarette smoke could also transiently inhibit and then enhance the phagocytic activity.
对长期暴露于香烟烟雾中的小鼠的吞噬功能进行了研究，并且还评估了体外暴露于香烟烟雾对巨噬细胞活性的影响。使用放射性标记的铜绿假单胞菌培养物来研究体内和体外的吞噬细胞活性。在工作日让小鼠暴露于新鲜香烟烟雾中长达37周，并测量其肺部的杀菌和清除活性。肺部清除和杀菌活性均受损。从暴露于烟雾的小鼠血液中静脉注射细菌的清除速率与对照小鼠相同，但肝脏中放射性标记物的积累减少。此外，肝脏中放射性标记物的消除速率低于对照。体外暴露于香烟烟雾的巨噬细胞最初吞噬率降低，但如果测量长时间的吞噬作用，最终其吞噬率会超过对照巨噬细胞。香烟烟雾的气相也可暂时抑制然后增强吞噬活性。
