Fick R B, Paul E S, Merrill W W, Reynolds H Y, Loke J S
Am Rev Respir Dis. 1984 Jan;129(1):76-81. doi: 10.1164/arrd.1984.129.1.76.
We studied the local response of the bronchoalveolar cell population after acute exposure to smoke from pyrolysis of Douglas fir wood. Cell viability experiments and function of the pulmonary macrophages were assessed, including adherence to surfaces, bacterial phagocytosis, and the intracellular bactericidal process. The smoke-exposed group (n=23) when compared with control animals (n=15) produced a significantly greater total cell yield (p less than 0.01) on bronchoalveolar lavage. There was no increase in the percentage of polymorphonuclear leukocytes. As carboxyhemoglobin levels increased, macrophage adherence to glass decreased (r = -0.79, p = 0.0001). Pulmonary macrophages from exposed animals were 33% less adherent at 120 min in vitro; however, these nonadherent smoke-exposed macrophages remained viable (55 to 78% viable by trypan blue dye exclusion). Both the phagocytic rate (361 14C cpm/unit time) and the maximal number of bacteria associated (at 60 min) with the exposed macrophages were significantly less than in the sham-exposed animals (uptake = 672 14C cpm/time, p less than 0.002 for each). The antibacterial properties of pulmonary macrophages are adversely affected by exposure to wood pyrolysis.
我们研究了急性暴露于花旗松木热解产生的烟雾后支气管肺泡细胞群体的局部反应。评估了细胞活力实验和肺巨噬细胞的功能，包括对表面的粘附、细菌吞噬作用以及细胞内杀菌过程。与对照动物（n = 15）相比，烟雾暴露组（n = 23）支气管肺泡灌洗的总细胞产量显著更高（p < 0.01）。多形核白细胞的百分比没有增加。随着碳氧血红蛋白水平的升高，巨噬细胞对玻璃的粘附减少（r = -0.79，p = 0.0001）。暴露动物的肺巨噬细胞在体外120分钟时的粘附性降低了33%；然而，这些未粘附的烟雾暴露巨噬细胞仍保持活力（通过台盼蓝染料排除法检测，活力为55%至78%）。暴露巨噬细胞的吞噬率（361 14C 计数/分钟）和（60分钟时）与细菌结合的最大数量均显著低于假暴露动物（摄取量 = 672 14C 计数/分钟，每项p < 0.002）。暴露于木材热解会对肺巨噬细胞的抗菌特性产生不利影响。
