Andersson H, Baechi T, Hoechl M, Richter C
Institute for Medical Radiobiology, University of Zurich, Switzerland.
J Microsc. 1998 Jul;191(Pt 1):1-7. doi: 10.1046/j.1365-2818.1998.00347.x.
We have investigated the autofluorescence of viable mammalian cells (DU-145 and V79) with a confocal laser scanning microscope equipped with a UV laser. Our aim was to investigate the autofluorescence dependence on different treatments in mitochondria and lysosomes by using different reagents and to improve the confocal laser scanning microscope image quality by deconvolution. The following conclusions were drawn from the results: (1) not all of the autofluorescence comes from mitochondria; (2) one can significantly affect the signal which comes from the mitochondria; (3) the other organelles involved are probably lysosomes; (4) it is harder to affect the autofluorescence signal from the lysosomes than that from the mitochondria, and (5) deconvoluted autofluorescence images provide better information than undeconvoluted ones.
我们使用配备紫外激光的共聚焦激光扫描显微镜研究了活的哺乳动物细胞（DU - 145和V79）的自发荧光。我们的目的是通过使用不同试剂研究线粒体和溶酶体中自发荧光对不同处理的依赖性，并通过去卷积提高共聚焦激光扫描显微镜的图像质量。从结果中得出以下结论：（1）并非所有自发荧光都来自线粒体；（2）可以显著影响来自线粒体的信号；（3）其他涉及的细胞器可能是溶酶体；（4）影响溶酶体的自发荧光信号比影响线粒体的更难；（5）去卷积后的自发荧光图像比未去卷积的图像提供更好的信息。
