Scully A D, Ostler R B, MacRobert A J, Parker A W, de Lara C, O'Neill P, Phillips D
Department of Chemistry, Imperial College of Science, Medicine and Technology, London, UK.
Photochem Photobiol. 1998 Aug;68(2):199-204.
Confocal fluorescence microscopy, using a newly constructed laser line-scanning confocal microscope, was applied to an investigation of the early stages of photoinduced destruction of V79-4 Chinese hamster fibroblasts using aluminum and zinc phthalocyanines as photosensitizers. Results obtained in this work show that aluminum and zinc phthalocyanines, once internalized, localize in perinuclear sites that are disrupted upon light exposure resulting in fluorescence redistribution. The combination of laser-line scanning with charge-coupled device detection used in the confocal microscope developed in this work can enable rapid high-resolution sequential imaging, which is ideal for studying photoinduced intracellular fluorescence dynamics.
利用新构建的激光线扫描共聚焦显微镜进行的共聚焦荧光显微镜检查，被应用于研究以铝酞菁和锌酞菁作为光敏剂对V79-4中国仓鼠成纤维细胞进行光诱导破坏的早期阶段。这项工作获得的结果表明，铝酞菁和锌酞菁一旦被内化，就会定位于核周部位，这些部位在光照后会被破坏，导致荧光重新分布。在这项工作中开发的共聚焦显微镜中使用的激光线扫描与电荷耦合器件检测相结合，能够实现快速高分辨率的序列成像，这对于研究光诱导的细胞内荧光动力学非常理想。
