Moan J, Berg K, Anholt H, Madslien K
Department of Biophysics, Institute for Cancer Research, Oslo, Norway.
Int J Cancer. 1994 Sep 15;58(6):865-70. doi: 10.1002/ijc.2910580620.
V79 cells incubated with di- or tetrasulfonated aluminium phthalocyanines (AlPcS2 or AlPcS4) showed a granular fluorescence pattern. Co-staining with the lysosomotropic dye acridine orange (AO) indicated that the granules that were stained by these photoactive phthalocyanines were identical to lysosomes. Small light exposures made the lysosomes permeable to the dyes without inactivating the cells. Also, the lysosomal enzymes beta-AGA and cathepsin (L+B) were inactivated by small light exposures when AlPcS4 was present. Such small and almost nontoxic light exposures caused a redistribution of the dyes in the cells that was accompanied by a more than 10-fold increase in the fluorescence quantum yields of the dyes. Surprisingly, this redistribution and increase in fluorescence did not result in any significant increase in the photosensitivity of the cells.
用二磺酸或四磺酸铝酞菁（AlPcS2或AlPcS4）孵育的V79细胞呈现出颗粒状荧光模式。与溶酶体亲和染料吖啶橙（AO）共染色表明，这些光活性酞菁染色的颗粒与溶酶体相同。小剂量光照使溶酶体对染料具有通透性，而不会使细胞失活。此外，当存在AlPcS4时，小剂量光照会使溶酶体酶β-AGA和组织蛋白酶（L+B）失活。这种小剂量且几乎无毒的光照导致染料在细胞内重新分布，同时染料的荧光量子产率增加了10倍以上。令人惊讶的是，这种重新分布和荧光增加并未导致细胞的光敏性有任何显著增加。
