García-Monzón C, Majano P L, Solís J A, Rodríguez S, Colina F, López-Botet M, Moreno-González E, Moreno-Otero R
Liver Unit, Hospital de la Princesa, Universidad Autónoma de Madrid, Spain.
Dig Dis Sci. 1998 Aug;43(8):1755-62. doi: 10.1023/a:1018835720267.
The mechanisms by which glucocorticoids are effective in acute liver rejection therapy are not entirely clear. The aims of this study were to characterize the intrahepatic immunological phenotype in acute liver rejection, as well as the effect of glucocorticoids on cytokine-stimulated hepatocyte cell lines. Biopsy sections from these patients were studied by immunohistochemistry. Cytokine-stimulated hepatocyte cell lines treated with glucocorticoids were evaluated by flow cytometry. The intrahepatic expression of both beta2-microglobulin conformational epitope and intercellular adhesion molecule-1 was higher in acute rejection than in resolving rejection. Interestingly, glucocorticoids were able to modulate in vitro the cytokine-induced expression of these molecules on hepatocyte cell lines. Beneficial effects of the glucocorticoid treatment appear to be associated with a modulation of a beta2-microglobulin conformational epitope and the intercellular adhesion molecule-1 on intrahepatic cellular targets in the acute rejection process.
糖皮质激素在急性肝移植排斥反应治疗中发挥作用的机制尚不完全清楚。本研究的目的是描述急性肝移植排斥反应时肝内的免疫表型，以及糖皮质激素对细胞因子刺激的肝细胞系的影响。通过免疫组织化学研究这些患者的活检切片。用流式细胞术评估经糖皮质激素处理的细胞因子刺激的肝细胞系。急性排斥反应时肝内β2-微球蛋白构象表位和细胞间黏附分子-1的表达均高于排斥反应缓解期。有趣的是，糖皮质激素能够在体外调节细胞因子诱导的这些分子在肝细胞系上的表达。糖皮质激素治疗的有益效果似乎与急性排斥反应过程中肝内细胞靶点上β2-微球蛋白构象表位和细胞间黏附分子-1的调节有关。
