Jorgensen S M, Demirkaya O, Ritman E L
Department of Physiology and Biophysics, Mayo Clinic, Rochester, Minnesota 55905, USA.
Am J Physiol. 1998 Sep;275(3):H1103-14. doi: 10.1152/ajpheart.1998.275.3.H1103.
A microcomputed tomography (micro-CT) scanner, which generates three-dimensional (3-D) images consisting of up to a billion cubic voxels, each 5-25 micron on a side, and which has isotropic spatial resolution, is described. Its main components are a spectroscopic X-ray source that produces selectable primary emission peaks at approximately 9, 18, or 25 keV and a fluorescing thin crystal plate that is imaged (at selectable magnification) with a lens onto a 2.5 x 2.5-cm, 1,024 x 1,024-pixel, charge-coupled device (CCD) detector array. The specimen is positioned close to the crystal and is rotated in 721 equiangular steps around 360 degrees between each X-ray exposure and its CCD recording. Tomographic reconstruction algorithms, applied to these recorded images, are used to generate 3-D images of the specimen. The system is used to scan isolated, intact, fixed rodent organs (e.g., heart or kidney) with the image contrast of vessel lumens enhanced with contrast medium. 3-D image display and analysis are used to address physiological questions about the internal structure-to-function relationships of the organs.
本文描述了一种微型计算机断层扫描（micro-CT）扫描仪，它能生成由多达十亿个立方体素组成的三维（3-D）图像，每个体素边长为5-25微米，且具有各向同性空间分辨率。其主要组件包括一个光谱X射线源，可产生约9、18或25 keV的可选一次发射峰，以及一个荧光薄晶体板，该晶体板通过透镜（以可选放大倍数）成像到一个2.5×2.5厘米、1024×1024像素的电荷耦合器件（CCD）探测器阵列上。样本靠近晶体放置，并在每次X射线曝光及其CCD记录之间围绕360度以721个等角步长旋转。应用于这些记录图像的断层重建算法用于生成样本的3-D图像。该系统用于扫描分离的、完整的、固定的啮齿动物器官（如心脏或肾脏），血管腔的图像对比度通过造影剂增强。3-D图像显示和分析用于解决有关器官内部结构与功能关系的生理问题。
