莫洛尼鼠白血病病毒基因组RNA的甲基化模式
Methylation pattern of genomic RNA from Moloney murine leukemia virus.
作者信息
Bondurant M, Hashimoto S, Green M
出版信息
J Virol. 1976 Sep;19(3):998-1005. doi: 10.1128/JVI.19.3.998-1005.1976.
Abstract
32P- and methyl-3H-labeled 70S Moloney murine leukemia virus RNA was purified from virions produced in Moloney murine leukemia virus-infected mouse embryo cells. Primer-free RNA subunits obtained by heat treatment and zonal centrifugation were digested with RNase T2, and methylated oligonucleotides were chromatographed on DEAE-Sephadex in 7 M urea. Approximately one molecule of RNase T2-stable oligonucleotide (-5 charge) was isolated per subunit. Structural analysis indicated that the sequence of the oligonucleotide is m7GpppGmpCp. Analysis of the mononucleotide fraction isolated by DEAE-Sephadex chromatography of the RNase T2 digest identified 15 to 23 internal N6-methyladenylic acid molecules per subunit.
摘要
从感染莫洛尼鼠白血病病毒的小鼠胚胎细胞产生的病毒粒子中纯化出用³²P和甲基³H标记的70S莫洛尼鼠白血病病毒RNA。通过热处理和区带离心获得的无引物RNA亚基用核糖核酸酶T2消化，甲基化寡核苷酸在含有7M尿素的二乙氨基乙基葡聚糖凝胶上进行层析。每个亚基大约分离出一个核糖核酸酶T2稳定的寡核苷酸分子（-5电荷）。结构分析表明，该寡核苷酸的序列为m⁷GpppGmpCp。对核糖核酸酶T2消化产物经二乙氨基乙基葡聚糖凝胶层析分离得到的单核苷酸部分进行分析，确定每个亚基含有15至23个内部N⁶-甲基腺苷酸分子。
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