腺病毒特异性RNA 5'端甲基化核苷酸序列。
Sequence of methylated nucleotides at the 5'-terminus of adenovirus-specific RNA.
作者信息
Moss B, Koczot F
出版信息
J Virol. 1976 Feb;17(2):385-92. doi: 10.1128/JVI.17.2.385-392.1976.
Abstract
RNA labeled with [methyl-3H] methionine and [14C]uridine was isolated from the cytoplasm of adenovirus-infected cells and purified by poly(U)-Sepharose chromatography and hybridization to filters containing immobilized adeovirus DNA. Analysis by dimethyl sulfoxide-sucrose gradient sedimentation suggested that the major mRNA species were methylated. 7-Methylguanosine was identified at the 5'-terminus of the advenovirus-specific RNA and could be removed by periodate oxidation and beta-elimination. Structures of the type m7G(5')ppp(5')Nm containing the unusual nucleoside N6, O2'-dimethyladenosine, and smaller amounts of 2'-O-methyladenosine were isolated by DEAE-cellulose chromatography after P1 nuclease digestion of the RNA. Evidence for some 5'-terminal sequences, m7G(5')ppp(5')m6AmpNm, with additional 2'-O-methylribonucleosides was also obtained. A base-methylated nucleoside, N6-methyladenosine, is located within the RNA chain and is released as a mononucleotide by alkali hydrolysis.
摘要
用[甲基 - ³H]甲硫氨酸和[¹⁴C]尿苷标记的RNA从腺病毒感染细胞的细胞质中分离出来，并通过聚（U） - 琼脂糖凝胶柱层析以及与固定有腺病毒DNA的滤膜杂交进行纯化。通过二甲基亚砜 - 蔗糖梯度沉降分析表明，主要的mRNA种类被甲基化。在腺病毒特异性RNA的5'末端鉴定出7 - 甲基鸟苷，并且可以通过高碘酸盐氧化和β - 消除反应将其去除。在RNA经P1核酸酶消化后，通过DEAE - 纤维素柱层析分离出了含有异常核苷N⁶，O²'-二甲基腺苷以及少量2'-O - 甲基腺苷的m⁷G(5')ppp(5')Nm类型的结构。还获得了一些具有额外2'-O - 甲基核糖核苷的5'末端序列m⁷G(5')ppp(5')m⁶AmpNm的证据。一种碱基甲基化的核苷，N⁶ - 甲基腺苷，位于RNA链内，并通过碱水解以单核苷酸形式释放出来。
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本文引用的文献
1
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Nucleic Acids Res. 1974 Jun;1(6):809-22. doi: 10.1093/nar/1.6.809.
2
Methylation of nucleic acids in HeLa cells.HeLa细胞中核酸的甲基化
Biochem Biophys Res Commun. 1965 Jul 26;20(3):298-302. doi: 10.1016/0006-291x(65)90363-3.
3
Adenovirus-associated virus multiplication. VI. Base compostion of the deoxyribonucleic acid strand species and strand-specific in vivo transcription.腺相关病毒的增殖。VI. 脱氧核糖核酸链种类的碱基组成及链特异性体内转录
J Virol. 1971 Nov;8(5):771-7. doi: 10.1128/JVI.8.5.771-777.1971.
4
On the lability of poly(A) sequences during extraction of messenger RNA from polyribosomes.关于从多核糖体中提取信使核糖核酸过程中多聚腺苷酸序列的不稳定性。
Biochim Biophys Acta. 1972 Mar 14;262(2):220-6. doi: 10.1016/0005-2787(72)90236-5.
5
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Eur J Biochem. 1972 Dec 4;31(2):246-54. doi: 10.1111/j.1432-1033.1972.tb02527.x.
6
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J Virol. 1972 Nov;10(5):909-19. doi: 10.1128/JVI.10.5.909-919.1972.
7
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