Makowska Krystyna, Obremski Kazimierz, Zielonka Lukasz, Gonkowski Slawomir
Department of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, ul. Oczapowskiego 13, 10-718 Olsztyn, Poland.
Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, ul. Oczapowskiego 13, 10-718 Olsztyn, Poland.
Toxins (Basel). 2017 Mar 10;9(3):98. doi: 10.3390/toxins9030098.
The enteric nervous system (ENS) can undergo adaptive and reparative changes in response to physiological and pathological stimuli. These manifest primarily as alterations in the levels of active substances expressed by the enteric neuron. While it is known that mycotoxins can affect the function of the central and peripheral nervous systems, knowledge about their influence on the ENS is limited. Therefore, the aim of the present study was to investigate the influence of low doses of zearalenone (ZEN) and T-2 toxin on calcitonin gene related peptide-like immunoreactive (CGRP-LI) neurons in the ENS of the porcine descending colon using a double immunofluorescence technique. Both mycotoxins led to an increase in the percentage of CGRP-LI neurons in all types of enteric plexuses and changed the degree of co-localization of CGRP with other neuronal active substances, such as substance P, galanin, nitric oxide synthase, and cocaine- and amphetamine-regulated transcript peptide. The obtained results demonstrate that even low doses of ZEN and T-2 can affect living organisms and cause changes in the neurochemical profile of enteric neurons.
肠神经系统（ENS）可响应生理和病理刺激而发生适应性和修复性变化。这些变化主要表现为肠神经元表达的活性物质水平的改变。虽然已知霉菌毒素会影响中枢和外周神经系统的功能，但关于它们对肠神经系统影响的知识有限。因此，本研究的目的是使用双重免疫荧光技术研究低剂量玉米赤霉烯酮（ZEN）和T-2毒素对猪降结肠肠神经系统中降钙素基因相关肽样免疫反应性（CGRP-LI）神经元的影响。两种霉菌毒素均导致所有类型肠丛中CGRP-LI神经元的百分比增加，并改变了CGRP与其他神经元活性物质（如P物质、甘丙肽、一氧化氮合酶以及可卡因和苯丙胺调节转录肽）的共定位程度。所得结果表明，即使是低剂量的ZEN和T-2也会影响生物体并导致肠神经元神经化学特征的变化。
