Wiesmann H P, Chi L, Stratmann U, Plate U, Fuchs H, Joos U, Höhling H J
Klinik und Poliklinik für Mund- und Kiefer-Gesichtschirurgie der Universität Münster, Waldeyerstrasse 30, D-48129 Münster, Germany.
Cell Tissue Res. 1998 Oct;294(1):93-7. doi: 10.1007/s004410051159.
The application of transmission electron microscopy (TEM) and atomic-force microscopy (AFM) aid the acquisition of detailed structural information on the process of hard tissue formation. The sutural mineralization of rat calvaria is taken as a model for a collagen-related mineralization system. After cryofixation or chemical fixation an anhydrous tissue preparation technique with no staining procedures is used. The atomic-force microscope and the transmission electron microscope are used for structural analysis of the mineralizing region of the sutural tissue. With the application of AFM the collagen macroperiod is shown to be well represented in the unmineralized sutural tissue. At the mineralization front the collagen fibrils are found to be thickened and to change to a characteristic stacked platelet structure. Using TEM the macroperiod is faintly visible before mineral crystallites have formed and is more prominent after the apatite crystallization has started in the fibrils. In this step a needle-like structure of the newly formed apatitic crystals is visible.
透射电子显微镜（TEM）和原子力显微镜（AFM）的应用有助于获取硬组织形成过程的详细结构信息。大鼠颅骨的缝合矿化被用作胶原相关矿化系统的模型。在冷冻固定或化学固定后，使用不进行染色程序的无水组织制备技术。原子力显微镜和透射电子显微镜用于对缝合组织矿化区域进行结构分析。通过AFM的应用，发现胶原大周期在未矿化的缝合组织中表现良好。在矿化前沿，发现胶原纤维变厚并转变为特征性的堆叠片状结构。使用TEM，在矿物微晶形成之前，大周期隐约可见，而在原纤维中开始磷灰石结晶后则更为明显。在这一步骤中，可以看到新形成的磷灰石晶体呈针状结构。
