FokI的结构对DNA切割有影响。
Structure of FokI has implications for DNA cleavage.
作者信息
Wah D A, Bitinaite J, Schildkraut I, Aggarwal A K
机构信息
Structural Biology Program, Department of Physiology and Biophysics, Box 1677, 1425 Madison Avenue, Mount Sinai School of Medicine, New York, NY 10029, USA.
出版信息
Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10564-9. doi: 10.1073/pnas.95.18.10564.
Abstract
FokI is a member an unusual class of restriction enzymes that recognize a specific DNA sequence and cleave nonspecifically a short distance away from that sequence. FokI consists of an N-terminal DNA recognition domain and a C-terminal cleavage domain. The bipartite nature of FokI has led to the development of artificial enzymes with novel specificities. We have solved the structure of FokI to 2.3 A resolution. The structure reveals a dimer, in which the dimerization interface is mediated by the cleavage domain. Each monomer has an overall conformation similar to that found in the FokI-DNA complex, with the cleavage domain packing alongside the DNA recognition domain. In corroboration with the cleavage data presented in the accompanying paper in this issue of Proceedings, we propose a model for FokI DNA cleavage that requires the dimerization of FokI on DNA to cleave both DNA strands.
摘要
FokI是一类特殊的限制酶成员，它能识别特定的DNA序列，并在离该序列短距离处进行非特异性切割。FokI由一个N端DNA识别结构域和一个C端切割结构域组成。FokI的二分性质导致了具有新特异性的人工酶的开发。我们已经将FokI的结构解析到了2.3埃的分辨率。该结构揭示了一个二聚体，其中二聚化界面由切割结构域介导。每个单体的整体构象与在FokI-DNA复合物中发现的相似，切割结构域与DNA识别结构域并列堆积。与本期《会议论文集》随附论文中给出的切割数据相一致，我们提出了一个FokI DNA切割模型，该模型要求FokI在DNA上二聚化以切割两条DNA链。
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