Williams H K, Sanders D S, Jankowski J A, Landini G, Brown A M
Department of Oral Pathology, School of Dentistry and Hospital, The University of Birmingham, UK.
J Oral Pathol Med. 1998 Aug;27(7):308-17. doi: 10.1111/j.1600-0714.1998.tb01962.x.
The immunocytochemical expression of cadherins and catenins was examined during the process of oral carcinogenesis by comparing their expression in normal and dysplastic epithelium with primary and metastatic carcinomas. While control epithelium showed normal distribution for P and E cadherin and the catenins, in severe dysplasia P-cadherin was upregulated. In other cases and in carcinoma-in-situ adjacent to infiltrating carcinomas, membranous expression of the cadherins and catenins was reduced or lost. The changes in expression of E-cadherin and the catenins suggest that disruption of the E-cadherin/catenin complex is a late event associated with invasion. In primary carcinomas reduced membranous and cytoplasmic staining were observed for both cadherins and catenins. Abnormal localisation of E-cadherin occurred in the more superficial parts of the better differentiated carcinomas, suggesting abnormality to the E-cadherin complex(es). In contrast, membranous expression of cadherins and catenins was reduced or lost in the deep invasive margin of primary carcinomas and in most poorly differentiated carcinomas. For E-cadherin at least, this reduction appears associated with differentiation, invasion and possibly prognosis. Possible mechanisms involved for changes in expression of the cadherins and associated catenins and areas for further study are discussed.
通过比较钙黏蛋白和连环蛋白在正常、发育异常上皮以及原发性和转移性癌中的表达，研究了口腔癌发生过程中它们的免疫细胞化学表达情况。对照上皮中P-钙黏蛋白和E-钙黏蛋白以及连环蛋白呈正常分布，而在重度发育异常中P-钙黏蛋白上调。在其他病例以及浸润性癌旁的原位癌中，钙黏蛋白和连环蛋白的膜表达减少或缺失。E-钙黏蛋白和连环蛋白表达的变化表明，E-钙黏蛋白/连环蛋白复合物的破坏是与侵袭相关的晚期事件。在原发性癌中，观察到钙黏蛋白和连环蛋白的膜染色及胞质染色均减少。E-钙黏蛋白的异常定位出现在高分化癌的较浅表部位，提示E-钙黏蛋白复合物存在异常。相反，在原发性癌的深部浸润边缘以及大多数低分化癌中，钙黏蛋白和连环蛋白的膜表达减少或缺失。至少对于E-钙黏蛋白而言，这种减少似乎与分化、侵袭以及可能的预后相关。文中讨论了钙黏蛋白和相关连环蛋白表达变化可能涉及的机制以及有待进一步研究的领域。
