Tarantino L M, McClearn G E, Rodriguez L A, Plomin R
Intercollege Graduate Program in Genetics, The Pennsylvania State University, University Park 16802, USA.
Alcohol Clin Exp Res. 1998 Aug;22(5):1099-105.
An F2 intercross derived from C57BL/6 and DBA/2 progenitor inbred strains was used to test for replication of quantitative trait loci (QTLs) for alcohol preference nominated by a previous study using BXD recombinant inbred (RI) strains (Rodriguez et al., Alcohol. Clin. Exp. Res. 19:367-379, 1995). Fourteen provisional QTLs were nominated in the original RI study with a p < 0.05 criterion. In the present study, a genome scan (101 microsatellite markers) was conducted on an F2 population (n = 218). Three significant QTLs were detected on chromosomes 1, 4, and 9, and three suggestive QTLs were detected on chromosomes 2, 3, and 10. Of these six QTLs, four were consistent with the previous RI nominations. The replication rate of 28.6% (4 of 14) is in agreement with the results of simulation studies performed by Belknap et al. (Behav. Genet. 26:149-160, 1996) and supports the methodological argument for a multistage research design for nominating and replicating QTLs.
利用从C57BL/6和DBA/2祖代近交系衍生而来的F2杂交群体，对先前一项使用BXD重组近交（RI）系的研究（Rodriguez等人，《酒精临床与实验研究》19:367 - 379, 1995）所确定的酒精偏好数量性状基因座（QTL）进行复制检验。在最初的RI研究中，以p < 0.05为标准确定了14个暂定QTL。在本研究中，对一个F2群体（n = 218）进行了全基因组扫描（101个微卫星标记）。在第1、4和9号染色体上检测到3个显著QTL，在第2、3和10号染色体上检测到3个提示性QTL。在这6个QTL中，有4个与先前RI研究的定位一致。28.6%（14个中的4个）的复制率与Belknap等人（《行为遗传学》26:149 - 160, 1996）进行的模拟研究结果相符，并支持了关于提名和复制QTL的多阶段研究设计的方法学观点。
