Blagojević D, Buzadzić B, Korać B, Saicić Z S, Radojicić R, Spasić M B, Petrović V M
Department of Physiology, Institute for Biological Research, Belgrade, Yugoslavia.
J Environ Pathol Toxicol Oncol. 1998;17(3-4):241-50.
As seasonal hibernators, ground squirrels decrease their body temperature to 7 degrees C and hibernate during the winter. Maintenance at 30 degrees C prevents seasonal changes of body temperature and animals remain euthermic and active. We measured selenium (Se)-dependent glutathione peroxidase (GSH-Px), as well as the activity of other antioxidative components such as superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), and glutathione-S-transferase (GST) and the amount of low-molecular-weight antioxidants glutathione (GSH), ascorbic acid (AsA), and vitamin E (vit E) in spring, summer, and winter in ground squirrels continuously kept at a temperature of 30 degrees C. We examined liver and interscapular brown adipose tissue (IBAT) as thermogenic tissues, as well as the brain and the kidneys. During the winter, we found a decrease in enzymatic activity and an increase in the level of low molecular antioxidants in all tissues. Correlation analysis revealed a similarity in the composition of antioxidative defense (AD) among the tissues examined. The results obtained clearly demonstrated numerous correlative expressions of antioxidative components in this experimental model, especially of GSH-Px, suggesting the complexity of the system responsible for the maintenance of physiological homeostasis.
作为季节性冬眠动物，地松鼠会将体温降至7摄氏度并在冬季冬眠。维持在30摄氏度可防止体温出现季节性变化，动物保持正常体温且活跃。我们测量了持续饲养在30摄氏度的地松鼠在春季、夏季和冬季时，硒（Se）依赖性谷胱甘肽过氧化物酶（GSH-Px）以及其他抗氧化成分如超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽还原酶（GR）和谷胱甘肽-S-转移酶（GST）的活性，以及低分子量抗氧化剂谷胱甘肽（GSH）、抗坏血酸（AsA）和维生素E（vit E）的含量。我们检查了作为产热组织的肝脏和肩胛间棕色脂肪组织（IBAT），以及大脑和肾脏。在冬季，我们发现所有组织中的酶活性降低，低分子抗氧化剂水平升高。相关性分析揭示了所检查组织中抗氧化防御（AD）组成的相似性。所得结果清楚地表明，在该实验模型中抗氧化成分存在大量相关性表达，尤其是GSH-Px，这表明负责维持生理稳态的系统具有复杂性。
